We narrowed to 9,392 results for: CAG

Showing: 8661 - 8680 of 9392 results

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Lenti-sgMroh2b#2/Cre

Plasmid

#193220

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgMroh2b#2 (Heatr7b2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-RBMS3

Plasmid

#185557

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

RBMS3 gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-3

Plasmid

#180399

Purpose

Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-3 (Irak1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-1

Plasmid

#180397

Purpose

Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-1 (Irak1 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-HHIP

Plasmid

#185551

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting HHIP

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

HHIP gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-ADGRG6

Plasmid

#185554

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting ADGRG6

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

ADGRG6 gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 FDX1-2

Plasmid

#184477

Purpose

Lentivirus gRNA targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-M2WA-siRNAres_S

Plasmid

#147519

Purpose

Mammalian Expression of HsTNRC6A-M2WA-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

four non silent mutations A339T, R934G, D1289G, a…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-CIM1mut-siRNAres_R

Plasmid

#147413

Purpose

Mammalian Expression of HsTNRC6A-CIM1mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1mut-siRNAres (TNRC6A Human)

Expression

Mammalian

Mutation

three non silent mutations A339T, R934G, and K157…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-1

Plasmid

#180388

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-1 (Rps6ka2 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSico_U6-PLOD2 sgRNA3

Plasmid

#136458

Purpose

Expression of gRNA against human PLOD2

Depositor

Antonio Amelio

Article

Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

Insert

gRNA against human PLOD2 (PLOD2 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-NCOA7g2 (BB35)

Plasmid

#139453

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-L2

Plasmid

#122307

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1 (Hdac1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Neo-sgCRTC2-2

Plasmid

#138703

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPN431

Plasmid

#137869

Purpose

Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Empty Bridge Control Zfp462-Klf4SE

Plasmid

#127666

Purpose

Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

gRNAs 129, 135, 115, 117

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Rescue myc-PCDHGC5 mRNA

Plasmid

#122229

Purpose

Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Tags

9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

Expression

Mammalian

Mutation

Silent mutations are in five consecutive codons …

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

Plasmid

#113696

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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