We narrowed to 13,498 results for: Mpl

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pD649-HAsp-MICA-COMP5AP-AviTag-9xHis

Plasmid

#157289

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MICA (MICA Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

Plasmid

#128835

Purpose

Knock-in of TagBFP into mouse Esrrb locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tagBFP into Esrrb locus (Esrrb Mouse)

Use

Mouse Targeting

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 QQ4xSS #2

Plasmid

#127195

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #2 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_FL_D290V

Plasmid

#98663

Purpose

Expresses MBP-tagged full length hnRNPA2 with D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

D290V

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_FL_P298L

Plasmid

#104468

Purpose

express MBP-tagged full length hnRNPA2 P298L

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

P298L

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-Mettl3

Plasmid

#196262

Purpose

In vitro transcription/translation of HA-tagged wild-type Mettl3

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 (Mettl3 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO-HsSyx3

Plasmid

#179288

Purpose

E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Tags

His6 + SUMO + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 393-792 from accession number NP_0010361…

Promoter

T7

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Synaptotagmin-6 scFv [N270/47]

Plasmid

#190544

Purpose

Mammalian Expression of Synaptotagmin-6 scFV. Derived from hybridoma N270/47.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Synaptotagmin-6 (Mus musculus) recombinant scFV (Syt6 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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TrpV1 scFv [N221/17]

Plasmid

#182082

Purpose

Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16-HSF1 WT

Plasmid

#71726

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 VP16 AD (HSF1 Human, Budding Yeast, Herpes Simplex Virus protein VP16)

Expression

Mammalian

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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actin CP alpha 2 beta 2

Plasmid

#13452

Depositor

John Cooper

Article

Bhattacharya et al J Biol Chem. 2006 Oct 13. 281(41):31021-

Insert

capping protein alpha 2 beta 2 (mouse) (Capzb, Capza2 Mouse)

Expression

Bacterial

Mutation

none

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-VCAM1-COMP5AP-AviTag-9xHis

Plasmid

#157506

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VCAM1 (VCAM1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp8-HF_GC3opt

Plasmid

#157699

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLNHA-C1-HsNot1iso1_840-1605_X

Plasmid

#147997

Purpose

Mammalian Expression of HsNot1iso1_840-1605

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_840-1605 (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFRSF6B-COMP5AP-AviTag-9xHis

Plasmid

#157277

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF6B (TNFRSF6B Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Tom20-mChF-AIP(TE)

Plasmid

#61522

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutation

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP with TE mutation (see comments), Tom20, and m…

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pATP416-pluxO5-ptetO7-crtYBI

Plasmid

#165975

Purpose

Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Inserts

Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

C1281A, G1698A and C66A, C1359T

Promoter

Synthetic promoter (pluxO5), Synthetic promoter (…

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Rufy3 scFv [N483/26]

Plasmid

#190558

Purpose

Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 ShCavin1-EGFP

Plasmid

#187253

Purpose

Lentiviral expression of shRNA targeting Cavin1

Depositor

Alpha Yap

Article

Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

Insert

ShRNA for Cavin1 (Cavin1 Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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GFP SRBC

Plasmid

#68399

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Insert

SRBC (Cavin3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only