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pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

Plasmid

#63884

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Promoter

EF1a

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4 1-129

Plasmid

#71401

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 1-129 (HDAC4 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pEMS1958

Plasmid

#82569

Purpose

High copy number plasmid containing PR2/3:Con MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

PRS2/3:Con

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX4T-1 hSlp4-a V21A

Plasmid

#40064

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a V21A (SYTL4 Human)

Tags

GST

Expression

Bacterial

Mutation

Valine 21 to Alanine

Promoter

Tac

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pAG75 ARHGEF18 3'UTR mut

Plasmid

#12055

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N1 3'UTR mut (ARHGEF18 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG175 AK024929 3'UTR mut

Plasmid

#12033

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N8 3'UTR mut (LINC00341 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pEMS1978

Plasmid

#49110

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre-WPRE

Use

AAV

Expression

Mammalian

Promoter

C8ORF46

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV mouse Vav1DN IRES GFP

Plasmid

#107092

Purpose

Mammalian expression retroviral vector

Depositor

Martine Roussel

Article

Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

Insert

Vav1 dominant negative cDNA (Vav1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

AA 1-186

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST40-ARL6-V5-His6

Plasmid

#67443

Purpose

Mammalian expression of hArl6 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEMS2026

Plasmid

#49133

Purpose

AAV plasmid with TNNT1-B (Ple301) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple301-iCre

Use

AAV

Expression

Mammalian

Promoter

TNNT1-B

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pAG77 GLCCI1 3'UTR mut

Plasmid

#12062

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

GLCCI1 3'UTR mut (GLCCI1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST40-SARA-V5-His6

Plasmid

#67452

Purpose

Mammalian expression of hSara with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4C-V5-His6

Plasmid

#67423

Purpose

Expression of hArl4c with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4-V5-His6

Plasmid

#67419

Purpose

Expression of hArl4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pBullet-pm-n

Plasmid

#53077

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAG227 CORO1C 3'UTR mut

Plasmid

#12030

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

CORO1C 3'UTR mut (CORO1C Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG83 LRRK1 3'UTR mut

Plasmid

#12058

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N4 3'UTR mut (LRRK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAAVS-NDi-H1.2_WT-Halo

Plasmid

#247484

Purpose

Expresses H1.2 (WT) by tet-on system

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

TRE3G

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-VSVG-mScar3-10xSunTag

Plasmid

#240630

Purpose

Expresses VSV-G, C-terminally fused to mScarlet3 and 10 repeats of the SunTag (GCN4) peptide, to luminally tag VSV-G-induced extracellular vesicles for use in EV-FUSIM assay.

Depositor

Esther Nolte-'t Hoen

Article

van den Ende et al J Extracell Vesicles. 2026 Mar;15(3):e70

Insert

VSVG-mScarlet3-10xSunTag

Tags

mScarlet3 and 10xSunTag

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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mEmerald-myo1f

Plasmid

#249265

Purpose

Fluorescently tagged myosin 1f

Depositor

Mira Krendel

Article

Barger et al Nat Commun. 2019 Mar 19;10(1):1249. doi:

Insert

Myo1f (MYO1F Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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