We narrowed to 44,941 results for: cha

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pcDNA3.1-Zeo-mEGFP

Plasmid

#214145

Purpose

Expresses the monomeric EGFP in eukaryotic cells

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

mEGFP

Expression

Mammalian

Mutation

A207K L221K F223R

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Hygro

Plasmid

#167907

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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GST-OPTN

Plasmid

#171424

Purpose

For the purifcation of OPTN protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

OPTN (OPTN Human)

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI-pheS

Plasmid

#111611

Purpose

Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Insert

pheS

Expression

Bacterial

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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3120 pSFFV-neo Bcl-XL

Plasmid

#8749

Depositor

Stanley Korsmeyer

Article

Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Insert

Bcl-xl (BCL2L1 Human)

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

Plasmid

#176573

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Avitag, Hexahistidine tag, and mEGFP

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-6xHis mAb

Plasmid

#214952

Purpose

Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-6xHis mAb heavy chain
VNp-anti-6xHis mAb light chain

Tags

VNp6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mRuby2

Plasmid

#99114

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWB107

Plasmid

#68561

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMS114

Plasmid

#215677

Purpose

Empty repair plasmid for ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxN + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDS220

Plasmid

#34980

Purpose

Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb–mCherry

Expression

Yeast

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) MID49 4xMyc Hisx6

Plasmid

#44596

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

MiD49 (Mief2 Mouse)

Tags

4xMyc and 6xHis

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) MID51 4xMyc Hisx6

Plasmid

#44598

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

MiD51 (Mief1 Mouse)

Tags

4xMyc and 6xHis

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pEC2861 (pSpy1C-PgyrA_ffluc)

Plasmid

#218511

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PgyrA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

Plasmid

#45574

Purpose

Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

co I-Sce I

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pROSA26-CreERT2

Plasmid

#149437

Purpose

ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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