We narrowed to 13,184 results for: BASE
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Plasmid#227099PurposeESR1 receptor overexpression for luciferase and barcode assaysDepositorInsertESR1 (ESR1 Human)
ExpressionMammalianAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sc++
Plasmid#198545PurposeExpresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSDDepositorInsertSc++
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRRH
Plasmid#198540PurposeExpresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRRH
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pYFAC-CH2-ubi-M-pyrG
Plasmid#221132PurposeExpression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.DepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus and …PromoterPalcA-T1-PalcS/M-T2-PaldA expression cassette (al…Available SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5423_p3WJdB-glcO60
Plasmid#221194PurposeDNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor systemDepositorInsertT7 promoter-glcO60-3WJdB
UseSynthetic BiologyExpressionBacterialPromoterT7 promoter with downstream glcO60 operatorAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPP4
Plasmid#209966PurposeContains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmidsDepositorInsertPromoter (P40)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLP-EV
Plasmid#209973PurposeContains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmidsDepositorInsertCDS (mock)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346
Plasmid#217012PurposeExpression of CDK7 in insect cellsDepositorInsertCDK7 (S164D, T170E)_del327-346 (CDK7 Human)
TagsTEV cleavable his tagExpressionInsectMutation(S164D, T170E)_del327-346Available SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac dual_Human full length CDK7(W132R, S164D, T170E)
Plasmid#217013PurposeExpression of CDK7 in insect cellsDepositorInsertCDK7(W132R, S164D, T170E) (CDK7 Human)
TagsTEV cleavable his tagExpressionInsectMutationCDK7(W132R, S164D, T170E)Available SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
Plasmid#217014PurposeExpression of CDK2-7 chimera in E.coliDepositorTagsC3 protease cleavable GST tagExpressionBacterialMutationCDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)Available SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGM268
Plasmid#220181PurposeExpresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tagDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM295
Plasmid#220183PurposeExpresses exogenous wild-type SEC61B with a C-terminal sfGFP tagDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only