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pET - FnR062.Y78M

Plasmid

#84794

Purpose

bacterial expression of FnR.0.6.2-Y78M protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78M

Tags

6xHis

Expression

Bacterial

Mutation

Y78M

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78N

Plasmid

#84795

Purpose

bacterial expression of FnR.0.6.2-Y78N protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78N

Tags

6xHis

Expression

Bacterial

Mutation

Y78N

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78Q

Plasmid

#84797

Purpose

bacterial expression of FnR.0.6.2-Y78Q protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78Q

Tags

6xHis

Expression

Bacterial

Mutation

Y78Q

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
pET - FnR062.Y78T

Plasmid

#84800

Purpose

bacterial expression of FnR.0.6.2-Y78T protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78T

Tags

6xHis

Expression

Bacterial

Mutation

Y78T

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only
mCh-cpFRB1

Plasmid

#155389

Purpose

Express cpFRB1 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

cpFRB1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCh-cpFRB2

Plasmid

#159645

Purpose

Express cpFRB2 as part of a circularly permuted rapamycin-inducible dimerization system (cpRAPID); visualized by mCherry2

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

cpFRB2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-C2

Plasmid

#155350

Purpose

Express human YTHDF1(360-559) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (360-559)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4L6

Plasmid

#153157

Purpose

Expression of Flag tagged human FOXD4L6 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4L6 (FOXD4L6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE99

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-PHKG1-V5-miniTurbo_IDG-K

Plasmid

#158192

Purpose

Gateway destination clone of PHKG1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PHKG1-V5-miniTurbo (PHKG1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-C1

Plasmid

#155349

Purpose

Express human YTHDF1(264-559) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (262-559)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF2-N

Plasmid

#155352

Purpose

Express human YTHDF2(1-381) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF2(1-381)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-N

Plasmid

#155351

Purpose

Express human YTHDF1(1-359) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (1-359)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST PHGDH

Plasmid

#154917

Purpose

Expresses human PHGDH

Depositor

Richard Possemato

Article

Richard et al Richard Possemato Lab plasmids

Insert

PHGDH (PHGDH Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA-fixed-length-clone-11

Plasmid

#120189

Purpose

Randomly picked clone 11 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA-fixed-length-clone-1

Plasmid

#120179

Purpose

Randomly picked clone 1 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-CENPBpA-fixed-length-clone-2

Plasmid

#120180

Purpose

Randomly picked clone 2 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-CENPBpA-fixed-length-clone-13

Plasmid

#120191

Purpose

Randomly picked clone 13 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-CENPBpA-fixed-length-clone-14

Plasmid

#120192

Purpose

Randomly picked clone 14 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-CENPBpA-fixed-length-clone-12

Plasmid

#120190

Purpose

Randomly picked clone 12 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-CENPBpA-fixed-length-clone-10

Plasmid

#120188

Purpose

Randomly picked clone 10 of the pCMV-GFP-CENPBpA-fixed-length library

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge