We narrowed to 12,197 results for: 112

1. pCRISPR-Cre stuffer v4

   Plasmid

   #158033

   Purpose

   Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTUB1:OsTIR1-3FLAG, SAG1:CAT

   Plasmid

   #87258

   Purpose

   TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

   Depositor

   David Sibley

   Article

   Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

   Inserts

   TIR1
   CAT
   

   Use

   Toxoplasma expression

   Tags

   3FLAG

   Mutation

   Codon optimized for Toxoplasma gondii expression

   Promoter

   TgSAG1 361 bp and TgTUB1 2717 bp

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG397

   Plasmid

   #226733

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   OMP25 (SYNJ2BP Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSICO-eGFP-TNPO3

   Plasmid

   #167590

   Purpose

   Expresses human TNPO3 fused to eGFP

   Depositor

   Zandrea Ambrose

   Article

   Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

   Insert

   eGFP-TNPO3 (TNPO3 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Mutation

   WT

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas12j-8-puro-crRNA

   Plasmid

   #194965

   Purpose

   The plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.

   Depositor

   Yongming Wang

   Article

   Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO human ULK2 shRNA 91

   Plasmid

   #27634

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   human ULK2 shRNA 91
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

   Plasmid

   #194693

   Purpose

   CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_06-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Promoter

   CMV

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194695

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV2-N-S202R

   Plasmid

   #177950

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (S202R) (N )
   

   Expression

   Mammalian

   Mutation

   S202R

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-PS-Grb2 (XSB363)

    Plasmid

    #78505

    Purpose

    Construct for expressing Grb2 in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Grb2 (GRB2 Human)
    

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. plenti2-EF1a-ChR2-EYFP-WRPE

    Plasmid

    #125255

    Purpose

    3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Roger D. Kamm

    Article

    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

    Insert

    hChR2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. tol2-GFAP::CD14

    Plasmid

    #59593

    Purpose

    Magnetic cell sorting of ES cell-derived astrocytes

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    human CD14 (CD14 Human)
    

    Expression

    Mammalian

    Promoter

    gfa2

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. MF84_PB-14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176628

    Purpose

    TF A self-activation cassette in MultiFate-2.3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    14xUAS-6x42bs-6x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dLight1.5

    Plasmid

    #111058

    Purpose

    Expresses dLight1.5 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.5
    

    Tags

    Flag tag

    Expression

    Mammalian

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-TSF-TEVcs-ULK1K46I

    Plasmid

    #171417

    Purpose

    For the purifcation of human ULK1 kinase dead complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Expression

    Mammalian

    Mutation

    kinase dead

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas3-Km

    Plasmid

    #207999

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCRISPR-CMV-cGAS3

    Plasmid

    #102610

    Purpose

    CRISPR/Cas9 lentivector targeting human cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    gRNA targeting human cGAS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MRGPRD-DuET

    Plasmid

    #213340

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRD (MRGPRD Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MiniCoopR U6:gRNA spred1, mitfa:Cas9

    Plasmid

    #118843

    Purpose

    Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    spred1 gRNA (spred1 Zebrafish)
    

    Use

    CRISPR; Tol2

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPRi(v1)-Blast

    Plasmid

    #170067

    Purpose

    Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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