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pRSFDuet1-tglA(C51)HIB

Plasmid

#134675

Purpose

Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Inserts

TglA(C51)
TglH
TglI
TglB

Tags

His6

Expression

Bacterial

Mutation

insertion of cysteine 51

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).LC_v1

Plasmid

#133083

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Expression

Bacterial

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).HC_v1

Plasmid

#133084

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Expression

Bacterial

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab3ab

Plasmid

#109636

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab3ab

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3ab (rab3ab Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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ORF18(R158A_L159A)-3xFLAG pCDNA4

Plasmid

#129814

Purpose

Expresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 158 to Alanine and Leucine 159 t…

Promoter

CMV

Available Since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72K

Plasmid

#127683

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72K

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72K

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72K

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G228A)-3xFLAG pCDNA4

Plasmid

#129809

Purpose

Expresses KSHV ORF18 (G228A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 228 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L29A)-3xFLAG pCDNA4

Plasmid

#129777

Purpose

Expresses KSHV ORF18 (L29A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 29 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L37A)-3xFLAG pCDNA4

Plasmid

#129779

Purpose

Expresses KSVH ORF18 (L37A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 37 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(K75A)-3xFLAG pCDNA4

Plasmid

#129784

Purpose

Expresses KSHV ORF18 (K75A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 75 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G65A)-3xFLAG pCDNA4

Plasmid

#129781

Purpose

Expresses KSHV ORF18 (G65A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 65 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L107A)-3xFLAG pCDNA4

Plasmid

#129787

Purpose

Expresses KSHV ORF18 (L107A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 107 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(W170)-3xFLAG pCDNA4

Plasmid

#129803

Purpose

Expresses KSHV ORF18 (W170A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Tryptophan 170 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G152A)-3xFLAG pCDNA4

Plasmid

#129800

Purpose

Expresses KSHV ORF18 (G152A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 152 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L151A)-3xFLAG pCDNA4

Plasmid

#129799

Purpose

Expresses KSHV ORF18 (L151A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 151 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L151A_G152A)-3xFLAG pCDNA4

Plasmid

#129813

Purpose

Expresses KSHV ORF18 (L151A_G152A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 151 to Alanine and Glycine 152 to…

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L191A)-3xFLAG pCDNA4

Plasmid

#129807

Purpose

Expresses KSHV ORF18 (L191A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 191 to Alanine

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(E36A_L37A)-3xFLAG pCDNA4

Plasmid

#129810

Purpose

Expresses KSHV ORF18 (E36A_L37A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glutamic acid 37 to Alanine and Leucine 3…

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G145A_N146A)-3xFLAG pCDNA4

Plasmid

#129812

Purpose

Expresses KSHV ORF18 (G145A_N146A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 145 to Alanine and Asparagine 146…

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(W81A)-3xFLAG pCDNA4

Plasmid

#129785

Purpose

Expresses KSHV ORF18 (W81A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Tryptophan 81 to Alanine

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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