We narrowed to 18,897 results for: Met;

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U6-(BbsI)sgRNA_CAG-Venus-bpA

Plasmid

#86985

Purpose

For cloning and expression of sgRNA together with Venus expression

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Venus

Expression

Mammalian

Mutation

Venus GFP variant

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-WT

Plasmid

#240057

Purpose

Express AAV2 WT capsid

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Insert

AAV2-WT Rep Cap

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAID 1.1 N-T2A-Bsr

Plasmid

#105985

Purpose

To make an auxin inducible degron mutant

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

Insert

T2A-Bsr

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

Plasmid

#173461

Purpose

C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCyterm-mScarlet-I3_N1

Plasmid

#189777

Purpose

Construct for testing oligimerization of mScarlet-I3 in mammalian cells by OSER assay

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

Cyterm-mScarlet-I3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCC25

Plasmid

#177319

Purpose

Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

Depositor

Rafal Tomecki

Article

Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

Insert

TtCutA 240-603

Tags

6xHis-SUMOTag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-ATP

Plasmid

#193819

Purpose

CMV overexpression of ChemoB-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-ATP

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGABASnFR.F102G

Plasmid

#112173

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

GFAP

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::ZeoR-bGHpA

Plasmid

#62441

Purpose

MXS_chaining vector with CMV::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP mCherry-ApoB-EGFP

Plasmid

#112859

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

mCherry-apoB-EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-Dectin1A-C-10

Plasmid

#57367

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Dectin1A (CLEC7A Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-CMV-CD4-sTb(C)-M13-GFP

Plasmid

#219780

Purpose

sTb(C) half CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTb(C)-M13-GFP

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Twist-siRNA3

Plasmid

#1784

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Expression

Mammalian

Available Since

April 28, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn-FLEX.iGABASnFR.F102G

Plasmid

#112164

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-HA LIP

Plasmid

#15739

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
PKAcs-GGSD5X-GFP

Plasmid

#108584

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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hum sgk promoter F4R4

Plasmid

#83426

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Article

Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

Insert

human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)

Use

Luciferase

Tags

Luciferase

Promoter

sgk

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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Puro-T2A-mCitrine-hSmad2-donor

Plasmid

#203663

Purpose

Donor plasmid for CRISPR knock-in

Depositor

Ali Hemmati Brivanlou

Article

Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

Insert

Smad2

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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His-Tev-WWP2

Plasmid

#186807

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-WWP2 (WWP2 Human)

Tags

His6-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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