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HOXB13 (human) Myc-tag-Bluescript

Plasmid

#8684

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Tags

Myc

Expression

Bacterial

Mutation

Myc tag on N-terminal

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
SIN40C.SFFV.dCas9-KRAB.P2A.mNeon

Plasmid

#170482

Purpose

Lentiviral dSpCas9-KRAB (CRISPRi) delivery, mNeon coexpression

Depositor

Dirk Heckl

Article

Ng et al iScience. 2023 Sep 7;26(10):107844. doi:

Inserts

dCas9-KRAB
SFFV
P2A-mNeonGreen

Use

Lentiviral

Tags

HA

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-sgRNA048_Grb10

Plasmid

#232934

Purpose

Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Grb10 S. pyogenes)

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMOD_A-CmYLCV-dCas9-24XGP41

Plasmid

#202009

Purpose

Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo level 1 vector

Tags

GS linkers

Promoter

CmYLCV

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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lentiguide-puro-wt-ltr

Plasmid

#182635

Purpose

Expresses guide RNA, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

S. pyogenes sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID3S

Plasmid

#188646

Purpose

Expresses Target-AID3S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

Plasmid

#149346

Purpose

Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

Cas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbc

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHygroMod-Foxd3-3xFlag

Plasmid

#37274

Depositor

Patricia Labosky

Insert

Foxd3 (Foxd3 Mouse)

Use

Mouse Targeting; Insertion vector for rmce

Tags

Flag tag on Foxd3

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
Donor idCas9-VP64-Blast

Plasmid

#255021

Purpose

Donor plasmid targeting the AAVS1 human locus to express dCas9-VP64 upon dox induction

Depositor

Danwei Huangfu

Article

Pulecio et al Cell Genom. 2026 Mar 18:101189. doi: 10.

Insert

dCas9-VP64

Tags

3x FLAG

Expression

Mammalian

Promoter

Tight TRE

Available Since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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ARL13B-(ΔGTPase)- GFP

Plasmid

#246843

Purpose

Express Arl13B (resistance to sgRNA) with deletion of GTPase, tagged with GFP

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted GTPase domain

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDYSCKO

Plasmid

#120263

Purpose

Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

Depositor

Christian Landry

Article

Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

Insert

DYSCKO cassette

Use

CRISPR and Synthetic Biology

Promoter

SNR52

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSPL10p::SPL10-GFP

Plasmid

#27406

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 (AT1G27370 Mustard Weed)

Use

Used to create transgenic lines

Tags

GFP

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only

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KIF3B

Plasmid

#25254

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KIF3B:S6-Q359 (KIF3B Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids S6-Q359

Available Since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
p70rg 5

Plasmid

#17829

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
Donor idCas9-KRAB-Blast

Plasmid

#255020

Purpose

Donor plasmid targeting the AAVS1 human locus to express dCas9-KRAB upon dox induction

Depositor

Danwei Huangfu

Article

Pulecio et al Cell Genom. 2026 Mar 18:101189. doi: 10.

Insert

dCas9-KRAB

Tags

3x FLAG

Expression

Mammalian

Promoter

Tight TRE

Available Since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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pSA127 A2UCOE-Cas9-BFP

Plasmid

#249151

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-Cas9-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEC3111 (pSpy1C-Plac(3)_ffluc)

Plasmid

#218778

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of mrfp cassette, mutagenesis of the RBS…

Promoter

Plac(3)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-NLS-6xHis

Plasmid

#207382

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG2_nat

Plasmid

#184914

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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