Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB

Plasmid

#148372

Purpose

Insect Expression of Dm4E-T-F41DW42D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-F41DW42D (4E-T Fly)

Expression

Insect

Mutation

5 non silent mutation L127S, E493G, H628R, N659S,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-del28-43-V5His6_Z

Plasmid

#148157

Purpose

Insect Expression of Dm4E-T_1-511-del28-43

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-del28-43 (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z

Plasmid

#148158

Purpose

Insect Expression of Dm4E-T_1-511-FWKtoAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-FWKtoAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-L36A L69A-V5His6_Z

Plasmid

#148159

Purpose

Insect Expression of Dm4E-T_1-511-L36AL69A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-L36AL69A (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-Y10AL15A-V5His6_Z

Plasmid

#148161

Purpose

Insect Expression of Dm4E-T_1-511-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-Y10AL15A (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z

Plasmid

#148162

Purpose

Insect Expression of Dm4E-T_1-511-YLLLtoAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-YLLLtoAAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-4mut-siRNAres_E

Plasmid

#146193

Purpose

Insect Expression of DmEDC3-4mut-siRNAres

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-4mut-siRNAres (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-5mut-siRNAres_E

Plasmid

#146194

Purpose

Insect Expression of DmEDC3-5mut-siRNA res

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-5mut-siRNA res (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-ADF_E

Plasmid

#146195

Purpose

Insect Expression of DmEDC3-ADF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-ADF (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-FAF_E

Plasmid

#146199

Purpose

Insect Expression of DmEDC3-FAF

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-FAF (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-FDA_E

Plasmid

#146200

Purpose

Insect Expression of DmEDC3-FDA

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmEDC3-FDA (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg6-sh(t10)RNAres_S

Plasmid

#147460

Purpose

Mammalian Expression of HsSmg6-sh(10)RNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg6-sh(10)RNAres (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7-siRNAres-shRNAres_S

Plasmid

#147463

Purpose

Mammalian Expression of HsSmg7-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres-shRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-G100E-siRNAres-V5-SBP-MBP_S

Plasmid

#147472

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-K66ER163E-siRNAres-V5-SBP-MBP_S

Plasmid

#147473

Purpose

Mammalian Expression of HsSmg7-K66ER163E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-K66ER163E-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-siRNAres-V5-SBP-MBP_S

Plasmid

#147474

Purpose

Mammalian Expression of HsSmg7-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-AvrII-dsRNAres_L

Plasmid

#146826

Purpose

Insect Expression of DmGW182-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-dsRNAres_L

Plasmid

#146831

Purpose

Insect Expression of DmGW182-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-515_I

Plasmid

#146566

Purpose

Bacterial Expression of HsSmg6_1-515

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-515 (SMG6 Human)

Expression

Bacterial

Mutation

two silent mutations and two non silent mutation …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pENmRFPCNAL2 pc1054

Plasmid

#167567

Purpose

The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

Depositor

Cristina Cardoso

Article

Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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