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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,364 results for: NIG

Showing: 861 - 880 of 2364 results

  2. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Perng-Kuang Chang
    Article
    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046
    Insert
    yA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    191015-plasmid-map-sequence-id-382132

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  3. Tet-pLKO-neo

    Plasmid
    #21916
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21916-plasmid-map-sequence-id-358974

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  11. pFlp-Gm

    Plasmid
    #222335
    Purpose
    Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3
    Depositor
    Philip Poole
    Article
    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10
    Inserts
    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    Use
    Tags
    Expression
    Mutation
    Promoter
    cl, flp, and sacB
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    222335-plasmid-map-sequence-id-451464

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  14. pCAGGS_NP_EBOV

    Plasmid
    #103049
    Purpose
    Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Article
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Insert
    NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    silent mutation (T to C) at position 928 of NP ge…
    Promoter
    CAG
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    103049-plasmid-map-sequence-id-197238

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  15. pFlp-Km

    Plasmid
    #222334
    Purpose
    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3
    Depositor
    Philip Poole
    Article
    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10
    Inserts
    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    Use
    Tags
    Expression
    Mutation
    Promoter
    cl, flp, and sacB
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    222334-plasmid-map-sequence-id-442294

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  19. pMP71-super-IL-2

    Plasmid
    #174599
    Purpose
    Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab
    Depositor
    Stanley Riddell
    Article
    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441
    Insert
    extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    174599-plasmid-map-sequence-id-344458

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  20. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Article
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112065-plasmid-map-sequence-id-217190

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Showing: 861 - 880 of 2364 results