We narrowed to 1,234 results for: cfp
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Plasmid#92314PurposeExpression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-deGFP-6xHis-T500
Plasmid#92222PurposeExpression of deGFP using the p70b promoter (weak version of the p70a promoter)DepositorInsertdeGFP
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500
Plasmid#92316PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p40651-EFSNS-FMR1_e1-24xms2
Plasmid#222968PurposeMS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequenceDepositorInsertFMR1-31CGG 5' UTR and exon1
ExpressionMammalianAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-RGS7
Plasmid#55760PurposeAn amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertmCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
TagsCer(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationRGS7 was amplified via PCR, which added an N-term…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 CA
Plasmid#133264PurposeConstitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.DepositorInsertOsRac1(G19V) (LOC4325879 Oryza sativa)
TagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 G19VPromoterUbiAvailable SinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 DN
Plasmid#133263PurposeDominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assaysDepositorInsertOsRac1(T24N) (LOC4325879 Oryza sativa)
TagsCFP-lipid and Venus-CRIBExpressionPlantMutationOsRac1 T24NPromoterUbiAvailable SinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)
Plasmid#184334PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500
Plasmid#92225PurposeExpression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue Y35 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p40651-EFSNS-FMR1_e1ΔCGG-24xms2
Plasmid#222969PurposeMS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequenceDepositorInsertFMR1-ΔCGG 5' UTR and exon1
ExpressionMammalianMutation31 CGG repeats deleted from 5' UTRAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500
Plasmid#92315PurposeExpression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL) mutant K16TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500
Plasmid#92227PurposeExpression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG D193TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidues Y35 D193 were mutated into TAG (Amber) s…Promoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92319PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92318PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92229PurposeExpression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500
Plasmid#92230PurposeExpression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue V86 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5082
Plasmid#85121PurposePkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization expDepositorInsertPkaiB-KaiC-ECFP
TagsECFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5085
Plasmid#85122PurposePkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.DepositorInsertPkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
TagsECFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps
Plasmid#181956PurposeGenetically encoded FRET-based cAMP indicator fused to AKAP79.DepositorInsertAKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
TagsAKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…ExpressionMammalianPromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only