We narrowed to 9,182 results for: Pol

1. pcDNA6-N-3XFLAG-Dvl2

   Plasmid

   #123587

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   dishevelled segment polarity protein 2 (Dvl2 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1 human full-length CENP-E-mNeon-6xHis

   Plasmid

   #180484

   Purpose

   pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

   Depositor

   Julie Welburn

   Article

   Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

   Insert

   CENP-E (CENPE human, Human, Synthetic)
   

   Tags

   mNeon-6xHis

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. KAT14-pDEST10

   Plasmid

   #118382

   Purpose

   HIS-tagged insect cell expression vector with KAT14.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   KAT14 (KAT14 Human)
   

   Tags

   HIS

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFB1.HMBP.PrS.PHF1

   Plasmid

   #125167

   Purpose

   Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   PHF1 (PHF1 Human)
   

   Tags

   His-MBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-Syn-NS1

   Plasmid

   #175276

   Purpose

   AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   NLS-dTomato (P2A cleavage)

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ratiometric FPX sensor for ERK kinase activity

   Plasmid

   #60974

   Purpose

   For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A-WW domain-ERK substrate-ddFP B
   

   Tags

   ERK substrate is before ddFP copy B, WW domain is…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLJM1 flag-cFLIP

   Plasmid

   #211529

   Purpose

   Expresses flag-tagged cFLIP (CFLAR)

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   cFLIP (CFLAR Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

   Plasmid

   #208041

   Purpose

   Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   TurboID
   

   Use

   Lentiviral

   Promoter

   EFS

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-TRE-NS1-dTomato

   Plasmid

   #175278

   Purpose

   AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   NS1; dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   dTomato (from bidirectional promoter)

   Promoter

   bidirectional TRE promoter

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB-GST-IRP2

    Plasmid

    #226621

    Purpose

    Expresses human IRP2 in insect cells

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Insert

    IRP2 (IREB2 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-shLuc.mKO2

    Plasmid

    #85224

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDUET-1-alpha-synuclein-mCerulean3-His6

    Plasmid

    #110061

    Purpose

    For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Insert

    alpha-synuclein (SNCA Human)
    

    Tags

    6 x His and mCerulean3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MmEos3.2

    Plasmid

    #203754

    Purpose

    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    Src15 n-term (SRC Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKE13-ISceI-fabp10a:CreERT2

    Plasmid

    #198242

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFAST-BAC BAF57/SMARCE1-His

    Plasmid

    #177861

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCE1 (SMARCE1 Human)
    

    Tags

    His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBacI-KDAC6

    Plasmid

    #224346

    Purpose

    Expresses human KDAC6 (HDAC6) in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC6 (HDAC6 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

    Plasmid

    #165445

    Purpose

    Expresses ACX, rPB(8kD) in mammalian cells

    Depositor

    Wei Leong Chew

    Article

    Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

    Insert

    APOBEC-nCas9-rPB(8kD)
    

    Expression

    Mammalian

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac1_GI.1_N116C-G193C

    Plasmid

    #162580

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Asparagine 116 was mutated to Cysteine and Glycin…

    Promoter

    polyhedrin

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dom neg CstF64

    Plasmid

    #127250

    Purpose

    Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    CstF64 delta 282 (CSTF2 Human)
    

    Use

    Other

    Expression

    Mammalian

    Mutation

    insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

    Promoter

    pEF1a

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid

    #89154

    Purpose

    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    

    Tags

    Intein and chitin binding domain

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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