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pIND20-KNL1-s24a_s60a-dCas9

Plasmid

#248563

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-s24a_s60a-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 S24A, S60A; Cas9 D10A, H840A

Promoter

TRE, miniCMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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UPF1-N-term-AID-V5-P2A-Hygro

Plasmid

#251490

Purpose

AID N-terminal UPF1 targeting vector

Depositor

Markus Landthaler

Article

Boehm et al Mol Cell. 2025 Sep 18;85(18):3524-3546.e

Insert

Homology arms for N-terminal targeting of UPF1 with AID-Hygro cassette

Tags

auxin-induced degron tag

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPF1-N-term-AID-V5-P2A-BSD

Plasmid

#251489

Purpose

AID N-terminal UPF1 targeting vector

Depositor

Markus Landthaler

Article

Boehm et al Mol Cell. 2025 Sep 18;85(18):3524-3546.e

Insert

Homology arms for N-terminal targeting of UPF1 with AID-BSD cassette

Tags

auxin-induced degron tag

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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px458-Cas9 UPF1 sgRNA

Plasmid

#251491

Purpose

Cas9 CRISPR gRNA construct; expresses human UPF1 gRNA

Depositor

Markus Landthaler

Article

Boehm et al Mol Cell. 2025 Sep 18;85(18):3524-3546.e

Insert

UPF1 gRNA

Expression

Bacterial

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2

Plasmid

#240441

Purpose

Sleeping Beauty vector for inducible expression of an shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA-NC1

Plasmid

#248557

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA-NC1 TD#277

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA-NC2

Plasmid

#248558

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA-NC2 TD#278

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA WT

Plasmid

#249271

Purpose

Expression of PKA (PRKACA) wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA13-3

Plasmid

#248540

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA13-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-Gal4UAS-GFP-PGK-BFP

Plasmid

#247506

Purpose

GAL4/UAS expression of GFP and constitutive expression of BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

GAL4/UAS expression of GFP and constitutive expression of BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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