We narrowed to 12,197 results for: 112

1. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

   Plasmid

   #50943

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Rose, Tobias Bonhoeffer

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Insert

   mRuby2-P2A-GCaMP6f
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWPT-/GCCACC-mEGFP-IRES-mCherry

   Plasmid

   #49235

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFast-Flag-hRad9

   Plasmid

   #222468

   Purpose

   Expresses hRad9 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad9 (RAD9A Human)
   

   Tags

   Flag epitope

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

   Plasmid

   #122333

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-AID-PcrA-UGI

   Plasmid

   #221220

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI
   

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD2148

   Plasmid

   #153968

   Purpose

   pCMV6-Entry vector encoding human IRAK1 protein 196F

   Depositor

   Dirk Dittmer

   Article

   Seltzer et al J Virol. 2020 May 4;94(10). pii: JVI.021

   Insert

   IRAK1 (IRAK1 Human)
   

   Tags

   Myc-DDK

   Expression

   Mammalian

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLC-EGFP-RIP3

   Plasmid

   #75165

   Purpose

   LentiCRISPR-EGFP with sgRNA targeting human RIP3

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Insert

   RIP3 sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD IFP1.4

   Plasmid

   #91775

   Purpose

   Bacterial expression of a fluorescent protein, Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (IFP1.4)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shu et al Science. 2009 May 8;324(5928):804-7. doi

   Insert

   Infrared Fluorescent Protein 1.4 + Heme oxygenase-1
   

   Tags

   Hexa-Histidine tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFast-hRad1

   Plasmid

   #222471

   Purpose

   Expresses hRad1 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad1 (RAD1 Human)
   

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

    Plasmid

    #78348

    Purpose

    Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-KRAB
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Promoter

    pEF alpha

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. hPU.1ΔN165-pET28b

    Plasmid

    #199796

    Purpose

    Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

    Depositor

    Gregory Poon

    Article

    Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

    Insert

    spi1 (SPI1 Human)
    

    Tags

    None

    Expression

    Bacterial

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

    Plasmid

    #122334

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

    Plasmid

    #194685

    Purpose

    CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-p38beta

    Plasmid

    #86833

    Purpose

    mammalian expression of GFP-p38beta

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38beta (MAPK11 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SSTR3-DuET

    Plasmid

    #213378

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR3 (SSTR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194687

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTorPE-R-GECO1

    Plasmid

    #32465

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    Substitutions relative to the mApple-derived anal…

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

    Plasmid

    #89150

    Purpose

    An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. OPN3-DuET

    Plasmid

    #213357

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPN3 (OPN3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-mGold-Calnexin-N-14

    Plasmid

    #158004

    Purpose

    Visualization of ER in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    Calnexin (CANX Human)
    

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    I474N

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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