We narrowed to 15,736 results for: LEA

1. pM.010

   Plasmid

   #228696

   Purpose

   C terminal Strep affinity tag with TEV cleavage site

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   TEV-Strep
   

   Expression

   Insect

   Mutation

   WT

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM.009

   Plasmid

   #228695

   Purpose

   N terminal Strep affinity tag with TEV cleavage site

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   Strep-TEV
   

   Expression

   Insect

   Mutation

   WT

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

   Plasmid

   #227086

   Purpose

   Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Mutation

   R1282H

   Promoter

   T7

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CUP1-∆ssCPY*-mScarletI

   Plasmid

   #221157

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Tags

   mScarletI

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CUP1-∆ssCPY*-mNeonGreen

   Plasmid

   #221156

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Tags

   mNeonGreen

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

   Plasmid

   #217324

   Purpose

   Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S969A

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

   Plasmid

   #217321

   Purpose

   Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S969A

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC-NHSTIIT-GG

   Plasmid

   #215789

   Purpose

   Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAc5.1B-EGFP-DmNot3-mut2-dsRNAres_Q

   Plasmid

   #147264

   Purpose

   Insect Expression of DmNot3-mut2-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3-mut2-dsRNAres (Not3 Fly)
   

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmNot3-F764A-dsRNAres_Q

    Plasmid

    #147280

    Purpose

    Insect Expression of DmNot3-F764A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-F764A-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-lambdaN-HA-DmNot3-F818A-dsRNAres_Q

    Plasmid

    #147281

    Purpose

    Insect Expression of DmNot3-F818A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-F818A-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-lambdaN-HA-DmNot3-mut1-dsRNAres_Q

    Plasmid

    #147282

    Purpose

    Insect Expression of DmNot3-mut1-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-mut1-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-lambdaN-HA-DmNot3-mut2-dsRNAres_Q

    Plasmid

    #147283

    Purpose

    Insect Expression of DmNot3-mut2-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-mut2-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-EGFP-DmNot3-F764A-dsRNAres_Q

    Plasmid

    #147261

    Purpose

    Insect Expression of DmNot3-F764A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-F764A-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-EGFP-DmNot3-mut1-dsRNAres_Q

    Plasmid

    #147263

    Purpose

    Insect Expression of DmNot3-mut1-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-mut1-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ppie.001.301.W257A

    Plasmid

    #137652

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Tara Davis, Melissa Jurica

    Insert

    ppie (PPIE Human)
    

    Tags

    GST-(His)6-thrombin

    Expression

    Bacterial

    Mutation

    001-301-W257A

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PRPF4.001.522.K45A.pc4

    Plasmid

    #137648

    Purpose

    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.

    Depositor

    Tara Davis

    Insert

    PRPF4 (PRPF4 Human)
    

    Tags

    NLS and myc-(His)6

    Expression

    Mammalian

    Mutation

    001-522-K45A-pc4

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PRPF4.001.522.Y32A.pc4

    Plasmid

    #137646

    Purpose

    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.

    Depositor

    Tara Davis

    Insert

    PRPF4 (PRPF4 Human)
    

    Tags

    NLS and myc-(His)6

    Expression

    Mammalian

    Mutation

    001-522-Y32A-pc4

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

    Plasmid

    #147262

    Purpose

    Insect Expression of DmNot3-F818A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-F818A-dsRNAres (Not3 Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C-BRPF3-8

    Plasmid

    #166516

    Purpose

    scFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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