We narrowed to 9,182 results for: Pol

1. pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7

   Plasmid

   #231352

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

   Plasmid

   #231353

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

   Plasmid

   #231356

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

   Plasmid

   #231357

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

   Plasmid

   #231358

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

   Plasmid

   #231359

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7

   Plasmid

   #231348

   Purpose

   Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7-SP6-BC(p11-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MKC177_CCR5(PGK-synEPOR)

   Plasmid

   #232415

   Purpose

   AAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.

   Depositor

   Kyle Cromer

   Article

   Shah et al bioRxiv 2024.04.12.589260

   Insert

   Truncated Erythropoietin Receptor (EPOR Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBacI-KDAC8

   Plasmid

   #224343

   Purpose

   Expresses human KDAC8 (HDAC8) in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

   Insert

   KDAC8 (HDAC8 Human)
   

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Feb. 26, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pFastBacI-KDAC6 H216A

    Plasmid

    #224351

    Purpose

    Expresses human KDAC6 (HDAC6) H216A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

    Insert

    KDAC6 (HDAC6 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 216 mutated to alanine

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pFastBacI-KDAC6 H611A

    Plasmid

    #224352

    Purpose

    Expresses human KDAC6 (HDAC6) H611A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

    Insert

    KDAC6 (HDAC6 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 611 mutated to alanine

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pMBS-849

    Plasmid

    #218004

    Purpose

    8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    8xHis-tag
    

    Use

    Synthetic Biology

    Tags

    PreScission protease cleavage site

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR-Sensor_P2A_SLC2A4

    Plasmid

    #221429

    Purpose

    Gateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteins

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    Sensor_P2A_SLC2A4 (SLC2A4 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVITRO1-HAPPID1

    Plasmid

    #204626

    Purpose

    Expresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.

    Depositor

    James McDonnell

    Article

    Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

    Inserts

    HAPPID1 heavy chain
    HAPPID1 light chain
    

    Expression

    Mammalian

    Promoter

    mouse elongation factor 1 alpha and rat elongatio…

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG416GAL10-HPeV_3C

    Plasmid

    #203465

    Purpose

    Expresses HPeV 3C protease from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    HPeV 3C protease (polyprotein gene )
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cox3-FusXTBE-Left TALE

    Plasmid

    #196504

    Purpose

    Expresses left-side TALE–DddAtox N term half cox3-FusXTBE in zebrafish

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    IDH2 MTS-cox3 left TALE-G1397 DddA-C-UGI-SV40 polyA
    

    Expression

    Bacterial

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cox1-FusXTBE-Left TALE

    Plasmid

    #196502

    Purpose

    Expresses left-side TALE–DddAtox N term half cox1-FusXTBE in zebrafish

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    IDH2 MTS-cox1 left TALE-G1397 DddA-C-UGI-SV40 polyA
    

    Expression

    Bacterial

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cox1-FusXTBE-RightTALE

    Plasmid

    #196503

    Purpose

    Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafish

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    IDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA
    

    Expression

    Bacterial

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cox3-FusXTBE-RightTALE

    Plasmid

    #196505

    Purpose

    Expresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafish

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    IDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA
    

    Expression

    Bacterial

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tl1-FusXTBE-Left TALE

    Plasmid

    #196506

    Purpose

    Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

    Depositor

    Stephen Ekker

    Article

    Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

    Insert

    IDH2 MTS-tl1 left TALE-G1397 DddA-C-UGI-SV40 polyA
    

    Expression

    Bacterial

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge