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5'-3xFLAG Mllt1-exon minimal CMV pCDNA5

Plasmid

#212066

Purpose

LR vector for integration of Mllt1-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mllt1-exon (Mllt1 Mouse)

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

Plasmid

#201964

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Publication

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E162A,M164A,R167A

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

Plasmid

#201968

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Publication

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

W255A,N262E,K266E

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-hMIGA2 (170-593)

Plasmid

#192833

Purpose

Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

Depositor

Karin Reinisch

Publication

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag- psa A Left -G1397N

Plasmid

#189645

Purpose

psa A Left -G1397N

Depositor

Jin-Soo Kim

Publication

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Expression

Plant

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

Plasmid

#136912

Purpose

DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

Depositor

Keith Joung

Publication

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

pCAG-DmrC-NLS-3xFLAG-VP64

Use

CRISPR

Expression

Mammalian

Promoter

Chicken Beta Actin

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1

Plasmid

#182376

Purpose

Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Publication

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

3xFlag
mito-mTFP1

Expression

Mammalian

Promoter

CMV promoter and SV40 promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

Plasmid

#182381

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Publication

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

Plasmid

#174133

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

Plasmid

#174135

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo R119A K120A nsp1

Plasmid

#175428

Purpose

Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Publication

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 R119A K120A

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants R119A K120A

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo E36A E37A nsp1

Plasmid

#175429

Purpose

Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Publication

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 E36A E37A

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants E36A E37A

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

Plasmid

#173167

Purpose

Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

Depositor

Kivanc Birsoy

Publication

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Calcineurin Like EF-Hand Protein 1 (CHP1 Human)

Use

Retroviral

Tags

3XFLAG

Mutation

Synonymous mutations at sgRNA sites, G2A, S6A

Promoter

MMLV

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro

Plasmid

#172633

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro

Plasmid

#158638

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Publication

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

E93Q/Q271A

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

Plasmid

#158639

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Publication

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q148A/Q271A

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG

Plasmid

#130680

Purpose

Expresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Publication

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_G1960del

Promoter

CMV

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG

Plasmid

#130677

Purpose

Expresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Publication

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

M1961_E1992del

Promoter

CMV

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG

Plasmid

#130689

Purpose

Expresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Publication

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-46.47A-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, M1961_E1992del

Promoter

CMV

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG

Plasmid

#130686

Purpose

Expresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Publication

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-9.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I378_G1960del

Promoter

CMV

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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