We narrowed to 16,131 results for: form

1. pOPINB-hRNF216(511-784)

   Plasmid

   #171923

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mGas8-PA

   Plasmid

   #176468

   Purpose

   Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   growth arrest specific 8 (Gas8 Mouse)
   

   Tags

   PA

   Expression

   Mammalian

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES2-EGFP-p53 tethered

   Plasmid

   #49245

   Purpose

   expresses two human WT p53s tethered together and also expresses EGFP in mammalian cells

   Depositor

   Dylan Taatjes

   Article

   Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

   Insert

   p53-p53 (TP53 Human)
   

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBK_Gal4-DBD-GR(NR3C1)

   Plasmid

   #227100

   Purpose

   GR receptor overexpression for luciferase and barcode assays

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   GR (NR3C1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pαH-S-RRAR/D614G

   Plasmid

   #164570

   Purpose

   SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR-D614G variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-CMV 3xFLAG-GABARAPL1-GFP

   Plasmid

   #123112

   Purpose

   Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_ER-RE_MLPmin_BC1065-luc2

   Plasmid

   #227111

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD40-His/V5-c-MycS62A

   Plasmid

   #45599

   Depositor

   Rosalie Sears

   Article

   Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

   Insert

   c-Myc S62A (Myc Mouse)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   S62A

   Promoter

   CMV

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pBabe puro H-Ras 61L

   Plasmid

   #13485

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   HRas 61L (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   61L

   Available Since

   Dec. 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pCEP4-BG505.SOSIP-gp160-QES.i05.c06

    Plasmid

    #123276

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcsII-pb4-flag

    Plasmid

    #86768

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 4 (PSMB4 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104095

    Purpose

    Expresses TREAT-siRNA reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. ASC-CASP1 Octamer

    Plasmid

    #164032

    Purpose

    Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CASP1, ASC (CASP1, PYCARD Human)
    

    Tags

    His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    W169G (ASC), G20K (CASP1)

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid

    #68218

    Purpose

    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc / Renilla / P19
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos / 35S / 35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104094

    Purpose

    Expresses TREAT reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

    Expression

    Mammalian

    Promoter

    TetCMV

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

    Plasmid

    #163988

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    M1457A

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV-hHOXA13

    Plasmid

    #135561

    Purpose

    Express hHOXA13 and EGFP in mammalian cells

    Depositor

    Jose Teixeira

    Article

    George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

    Insert

    Homeobox A13 (HOXA13 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cer(1-158)-RGS7

    Plasmid

    #55760

    Purpose

    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    mCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
    

    Tags

    Cer(1-158) was fused to the amino terminus of RGS…

    Expression

    Mammalian

    Mutation

    RGS7 was amplified via PCR, which added an N-term…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA6.2EmGFP-cDAXX

    Plasmid

    #169727

    Purpose

    Mammalian expression construct of GFP tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    cDAXX (DAXX Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Comprises residues 495-740

    Promoter

    CMV

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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