We narrowed to 16,183 results for: nes
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UHRF1
Plasmid#25359PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertubh12.672.793 (UHRF1 Human)
TagsHis with thrombin cleavage siteExpressionBacterialMutationamino acid residues 672-793.Available sinceDec. 21, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
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Donor idCas9-KRAB-Blast
Plasmid#255020PurposeDonor plasmid targeting the AAVS1 human locus to express dCas9-KRAB upon dox inductionDepositorInsertdCas9-KRAB
Tags3x FLAGExpressionMammalianPromoterTight TREAvailable sinceMay 20, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-SpCas9-HF1-NLS-6xHis
Plasmid#207382PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-24XGP41-J9
Plasmid#202006PurposeMoClo level 0 dCas9-24XGP41 coding sequence moduleDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-24XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEasyG2_nat
Plasmid#184914PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-eSpCas9
Plasmid#126769PurposeExpression of increased fidelity eSpCas9 in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT3TS-nCas9n-A71
Plasmid#125644Purposemicro-injection of poly-adenylated nCas9n RNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnCas9n
UseRna in vitro transcription vectorPromoterT3Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
USP08 (2GWF)
Plasmid#25217PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertusp08.hs.0001-0142 (USP8 Human)
TagsHisExpressionBacterialMutationrhodanese domain, residues 181-318Available sinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
M10.2::GFP-ACNF Targeting Vector
Plasmid#15859DepositorInsertM10.2
ExpressionBacterialAvailable sinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX Neo TagBFP-LC3B
Plasmid#250866PurposeStable lentiviral expression of TagBFP-LC3BDepositorPublicationAvailable sinceFeb. 25, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS
Plasmid#244834PurposeBacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpG Cas9
UseCRISPRTags2xSV40 NLS and 10xHis-MBPExpressionBacterialMutationD1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterT7Available sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRS-dCas9
Plasmid#220193Purposemini-Tn7 dCas9 expression vectorDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_zeo
Plasmid#184913PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_hph
Plasmid#184915PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_zeo
Plasmid#184917PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Hygro
Plasmid#186671PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Hygromycin (YTHDF2 Human)
ExpressionMammalianAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
ExpressionMammalianAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only
