We narrowed to 14,443 results for: NTS

1. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

   Plasmid

   #184335

   Purpose

   Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

   Depositor

   Peter Scheiffele

   Article

   Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

   Insert

   Nrxn3-alpha-GPI (Nrxn3 Mouse)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4999

   Plasmid

   #107555

   Purpose

   Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(dead1)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D917A

   Promoter

   CMV

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. 423Gal-FUS-YFP

   Plasmid

   #29615

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   YFP

   Expression

   Yeast

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)_Paragranulin-no linker

   Plasmid

   #176917

   Purpose

   express Paragranulin without linker 1 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Paragranulin (GRN Human)
   

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-PI(WT)-Rac1(WT)-mCherry

   Plasmid

   #87550

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive Rac1

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Rac1 (RAC1 Human)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX131

   Plasmid

   #167149

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   4'-phosphopantetheinyl transferase, npgA
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Δ18 P.2/B.1.1.248

   Plasmid

   #173477

   Purpose

   Encodes SARS-CoV-2 P.2/B.1.1.248 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 Spike Truncated to remove 18 amino acids
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-PI(WT)-Rac1(Q61L)-mCherry

   Plasmid

   #87553

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Rac1 (RAC1 Human)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Q61L

   Promoter

   CMV

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

   Plasmid

   #176535

   Purpose

   Non-protein coding locus components from CRISPR-associated IscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available Since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

11. tRNA-gRNA position [D1_n-1] (GB1208)

    Plasmid

    #75409

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [D1_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCHECK2-E2F3 3'UTR

    Plasmid

    #29470

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    E2F3 3'UTR (E2F3 Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1(+)_Granulin 2-no linker

    Plasmid

    #176921

    Purpose

    express Granulin 2 without linker 3 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 2 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)_Granulin 1+linker2

    Plasmid

    #176918

    Purpose

    express Granulin 1 with linker 2 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 1+linker2 (GRN Human)
    

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. psiCHECK2-CHEK1 3'UTR

    Plasmid

    #29478

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    CHEK1 3'UTR (CHEK1 Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

16. PET-SpCas9-HF1-plus-NLS-6xHis

    Plasmid

    #207381

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, Q695A, Q926A, amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-B-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207385

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1 ObLiGaRe Donor vector/EPB58

    Plasmid

    #90016

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by inverted ZFN binding sites (PPP1R12C Human)
    

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJO427

    Plasmid

    #111139

    Purpose

    URT-type vector for Glu-mIκBα expression in Mammalian cells

    Depositor

    Alexander Varshavsky

    Article

    Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_ZKSCAN1_Tau10-12WT

    Plasmid

    #194163

    Purpose

    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
    

    Tags

    3X flag tag

    Expression

    Mammalian

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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