We narrowed to 15,736 results for: LEA

1. pTU1-A-recJU

   Plasmid

   #112783

   Purpose

   Upstream homology arm, RecJ is a ssDNA specifiC exonuclease, involved in genome repair and maintenance

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   recJU
   

   Expression

   Bacterial

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2QNR (NEDD5)

   Plasmid

   #101273

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NEDD5 (SEPTIN2 Human)
   

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. 5C0M

   Plasmid

   #69962

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SGF29:JMC01N-E01:C207060 (SGF29 Human)
   

   Tags

   His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. 4Z31

   Plasmid

   #69958

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RC3H2:YTC020-E10:C234061 (RC3H2 Human)
   

   Tags

   His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. 4Z0O

   Plasmid

   #69954

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
   

   Tags

   His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. 4YVD

   Plasmid

   #69960

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PLRG1:JMC102-C09:C233452 (PLRG1 Human)
   

   Use

   Baculovirus expression

   Tags

   His and TEV cleavage site

   Promoter

   polyhedrin

   Available Since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. 4MYI

   Plasmid

   #60103

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PVX_084705:MAC04G-C11:C228845
   

   Use

   Baculovirus expression

   Tags

   6x His and TEV cleavage site

   Mutation

   See comments

   Promoter

   polyhedrin

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker

   Plasmid

   #50627

   Purpose

   pRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Lentiviral and TALEN

   Expression

   Mammalian

   Available Since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers

   Plasmid

   #50624

   Purpose

   pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.

   Depositor

   Andrew Scharenberg

   Type

   Empty backbone

   Use

   Mrna production

   Promoter

   T7

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRII-Topo Grp

    Plasmid

    #45871

    Depositor

    Jeffrey Macklis

    Insert

    GRP in situ probe (Grp Mouse)
    

    Use

    In situ

    Mutation

    nt 66-504 on NM_175012

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

11. RPRD2

    Plasmid

    #40740

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RPRD2 (RPRD2 Human)
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

12. MPP8 (3R93)

    Plasmid

    #32050

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MPP8 (MPHOSPH8 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pTH672-SUP35-Y351C

    Plasmid

    #29733

    Depositor

    Tobias von der Haar

    Insert

    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Y351C mutant gene of SUP35 including genomic sequ…

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-LaminB1-10

    Plasmid

    #55069

    Purpose

    Localization: Nuclear Envelope, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAT2

    Plasmid

    #112583

    Purpose

    Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid

    #174901

    Purpose

    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG and NES

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNIC-HIS6-MBP-TEV-GG

    Plasmid

    #215800

    Purpose

    Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pNIC-HIS6-NUSA-TEV-GG

    Plasmid

    #215798

    Purpose

    Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNIC-HIS10-ZBASIC-TEV-GG

    Plasmid

    #215797

    Purpose

    Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEGFP-C1 NFAT3

    Plasmid

    #10961

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    NFAT3 (NFATC4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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