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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,712 results for: NSI

Showing: 8861 - 8880 of 11712 results

  3. EGFP-p300 (D1399Y)

    Plasmid
    #191763
    Purpose
    Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    FRB-EGFP-p300 core (D1399Y) (EP300 Human)
    Tags
    EGFP (N-terminal of p300 core (D1399Y)) and FRB (…
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic D1399Y mutation in p300
    Promoter
    EF1alpha
    Available Since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191763-plasmid-map-sequence-id-383798

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  5. pMSCVpuro-Mito-DsRed1

    Plasmid
    #87379
    Purpose
    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    TXN2 mitochondrial transit peptide (TXN2 Human)
    Use
    Retroviral
    Tags
    DsRed1
    Expression
    Mammalian
    Mutation
    First 60 aa only
    Promoter
    Retroviral 5' LTR promoter
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87379-plasmid-map-sequence-id-212256

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  11. pLKO_HA.V5.FLAG_NGFR

    Plasmid
    #158250
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.FLAG
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158250-plasmid-map-sequence-id-327836

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  13. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available Since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    112060-plasmid-map-sequence-id-216934

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  15. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

    Plasmid
    #195503
    Purpose
    SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
    Use
    CRISPR; Donor vector
    Tags
    T2A-H2B-mCitrine
    Expression
    Mammalian
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195503-plasmid-map-sequence-id-384989

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  19. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191163
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP-3xMS2
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191163-plasmid-map-sequence-id-375409

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Showing: 8861 - 8880 of 11712 results