We narrowed to 9,182 results for: Pol

1. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV-EGFP.Flag-EZH2-mt1

   Plasmid

   #220244

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 1 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R27A, R31A, F165A

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

   Plasmid

   #160566

   Purpose

   GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   Multiplexing Edit (E3-E4-En-1)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFAST-BAC BAF155/SMARCC1-His

   Plasmid

   #177863

   Purpose

   Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

   Depositor

   Yoshihiro Izumiya

   Article

   Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

   Insert

   SMARCC1 (SMARCC1 Human)
   

   Tags

   His and His tag at C-terminus with GGGGS linker

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVtrunc Affimer6-msfGFPΔN12

   Plasmid

   #231550

   Purpose

   Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Affimer6
   

   Tags

   msfGFP

   Expression

   Mammalian

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVNP2.0-SpCas9

   Plasmid

   #206880

   Purpose

   Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Insert

   SpCas9 (cas9 )
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-2t

   Plasmid

   #159752

   Purpose

   CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   sgRNA
   

   Use

   CRISPR

   Available Since

   Oct. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCMVtrunc Affimer6-sfCherry2ΔN12

   Plasmid

   #231551

   Purpose

   Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Affimer6
   

   Tags

   sfCherry2

   Expression

   Mammalian

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastbac1-GST-hUSP7

   Plasmid

   #63573

   Purpose

   Expression of synthetic human USP7 tagged with GST

   Depositor

   Titia Sixma

   Article

   Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

   Insert

   USP7 (USP7 Human, Synthetic)
   

   Tags

   GST

   Expression

   Insect

   Mutation

   Synthetic based on human protein sequence (please…

   Promoter

   Polyhedrin

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB1.HMBP.PrS.JARID2

    Plasmid

    #125166

    Purpose

    Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    JARID2 (JARID2 Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-LN2

    Plasmid

    #188235

    Purpose

    Polycistronic human LIN28 and NANOG were cloned into the pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

    Inserts

    LIN28A (LIN28A Human)
    NANOG (NANOG Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac (GST-SNAP-Shp2)

    Plasmid

    #177897

    Purpose

    Baculo expression of GST tag fused to SNAP tag and human Shp2

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 (PTPN11 Human)
    

    Tags

    TEV-GST-PreScission cut site-SNAP tag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBacI-KDAC8 H143A

    Plasmid

    #224344

    Purpose

    Expresses human KDAC8 (HDAC8) H143A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC8 (HDAC8 Human)
    

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 143 mutated to alanine

    Promoter

    Polyhedrin

    Available Since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pHAGE2-TetOminiCMV-OS

    Plasmid

    #136576

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHIV-EGFP.Flag-EZH2-mt2*

    Plasmid

    #220246

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2* (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PRKKKR494-499NAAIRS

    Promoter

    EF-1α

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-miRE-shRNA IFT88-PGK-neo-IRES-GFP

    Plasmid

    #73576

    Purpose

    Mammalian retroviral expression vector

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    IFT88 (Ift88 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

    Plasmid

    #160568

    Purpose

    tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    M1_1-pTRNA-scf 2.1
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG416GAL10-WNV_NS2B-GS-NS3

    Plasmid

    #203475

    Purpose

    Expresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Article

    Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

    Insert

    WNV NS2B-GS-NS3 (POLY )
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid

    #75411

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [M1_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Hyg-mEGFP-pp4640

    Plasmid

    #191846

    Purpose

    Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusion

    Depositor

    Bertrand Collet

    Article

    Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

    Insert

    mEGFP-pp4640
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    mEGFP fusion in Nterminal, some silent point muta…

    Promoter

    CMV

    Available Since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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