We narrowed to 9,894 results for: EPO

1. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

   Plasmid

   #223209

   Purpose

   Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   IL10 (IL10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR-53BP1 1-1972 WT

   Plasmid

   #53446

   Purpose

   Recombinational donor/master vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   siRNA resistant and mutations A128V, T720A, A1347…

   Promoter

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1-nAChr-Alpha6-mCherry

   Plasmid

   #50480

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   mCherry fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA6.2-YFP-CWT

   Plasmid

   #176689

   Purpose

   Fluorescent reporter for phagosomal escape of Staphylococcus aureus

   Depositor

   Martin Fraunholz

   Article

   Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

   Insert

   cell wall-targeting domain of Lysostaphin
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   deletion of protase domain of Lysostaphin

   Promoter

   CMV

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-Chronos-GFP

   Plasmid

   #99232

   Purpose

   AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCAR_sgRNA_puro-mKate-lox5171

   Plasmid

   #162077

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LSB-hsa-miR-19a-3p

   Plasmid

   #103323

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-19a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-19a-3p target (MIR19A Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

   Plasmid

   #58959

   Purpose

   lentiviral expression of v-H-Ras and luciferase/EGFP reporter

   Depositor

   Snorri Thorgeirsson

   Article

   Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

   Inserts

   CMV enhancer/chicken beta actin promoter
   v-H-Ras
   luc2/EGFP fusion gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer/chicken beta actin

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-UBC-FLEX-XRI-FLAG

   Plasmid

   #178057

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-FLAG
   

   Use

   AAV

   Tags

   FLAG-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastbac1-GST-hUSP7

    Plasmid

    #63573

    Purpose

    Expression of synthetic human USP7 tagged with GST

    Depositor

    Titia Sixma

    Article

    Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

    Insert

    USP7 (USP7 Human, Synthetic)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Synthetic based on human protein sequence (please…

    Promoter

    Polyhedrin

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid

    #134653

    Purpose

    lysosome-targeted Sac1 catalytic domain C389S

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    

    Use

    Lentiviral

    Tags

    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…

    Expression

    Mammalian

    Mutation

    C389S and contains only amino acids 77-520 (Pleas…

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. hum alpha ENAC promoter a25-a23

    Plasmid

    #83427

    Purpose

    promoter reporter construct to test 5' flanking sequences

    Depositor

    Christie Thomas

    Article

    Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

    Insert

    human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    alpha ENAC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #105000

    Purpose

    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid

    #167971

    Purpose

    Tp63 reporter targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    Tp63 (TP63 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKrox24(MapERK)d1EGFP (KroxDs)

    Plasmid

    #214912

    Purpose

    for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    d1EGFP under the MapERK promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MapkERK promoter

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Halo-p58

    Plasmid

    #166896

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    p58 (Lman1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    L405Q, R419S- please see depositor comments

    Promoter

    CMV

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE

    Plasmid

    #85453

    Purpose

    Lentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-sgRNA-Backbone-UP-TdTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    deletion of AA123-365 in tdTomato (please see dep…

    Promoter

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

    Plasmid

    #202059

    Purpose

    Inducible AAV activator

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITRs intact

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO HIS HSPH2

    Plasmid

    #19535

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 4 (HSPA4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    mutations A381T, T732A, and an insertion of "…

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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