We narrowed to 16,131 results for: form

1. pIE-hα2M-H6

   Plasmid

   #128492

   Purpose

   Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (A2M Human)
   

   Tags

   His tag (6x)

   Expression

   Insect

   Promoter

   IE1

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIG AKT3 S472A

   Plasmid

   #73052

   Purpose

   Human AKT3 S472A

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 S472A (AKT3 Human)
   

   Expression

   Mammalian

   Mutation

   S472A

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-mKate-MKLP1

   Plasmid

   #140570

   Purpose

   Expresses mKate tagged MKLP1

   Depositor

   Heidi Hehnly

   Article

   Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

   Insert

   MKLP1 (KIF23 Human)
   

   Tags

   mKate

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-IY GBRPL1

   Plasmid

   #89294

   Purpose

   For expression of the mammalian Atg8 protein GABARAPL1 without a tag

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   GABARAPL1 (GABARAPL1 Human)
   

   Use

   Retroviral

   Tags

   IRES-YFP

   Expression

   Mammalian

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMX-IY HA-GBRPL1

   Plasmid

   #89297

   Purpose

   For expression of the mammalian Atg8 protein GABARAPL1 tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   GABARAPL1 (GABARAPL1 Human)
   

   Use

   Retroviral

   Tags

   HA and IRES-YFP

   Expression

   Mammalian

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH728-CEN-RLuc/maxCFLuc

   Plasmid

   #38212

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5-Flag-EPLIN alpha delta LIM

   Plasmid

   #40933

   Depositor

   Elizabeth Luna, Scott Maul

   Article

   Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

   Insert

   EPLIN alpha (LIMA1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   LIM domain deleted- amino acids 221-295

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pmx-MiGT

   Plasmid

   #172397

   Purpose

   Retroviral expression vector for murine direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

   Insert

   Mef2c-i, Gata4, Tbx5 (Gata4, Mef2c, Tbx5 Mouse)
   

   Use

   Retroviral

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPGK-T7/2-CD44v6-10 (-cyt)

   Plasmid

   #137821

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6-10 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121817

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid

    #192444

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast SHMT2 CD

    Plasmid

    #106302

    Purpose

    Expresses SHMT2 K280A catalytic site mutant

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    K280A catalytic site mutation, Silent mutations d…

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mitoTALECD for_atp1_1397CN

    Plasmid

    #186203

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2-mCherry-MKLP1

    Plasmid

    #140562

    Purpose

    Expresses mCherry tagged MKLP1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    MKLP1 (KIF23 Human)
    

    Tags

    2xmCherry

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid

    #121814

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. actc1b-mKate2-SERCA1

    Plasmid

    #109498

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    SERCA1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Mutation

    Please see Depositor Comments

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT-DHX36iso2E335A

    Plasmid

    #159588

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    E335A

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO_GFP_Luciferase

    Plasmid

    #155079

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-ms-c-Myc-V394D

    Plasmid

    #50775

    Purpose

    retroviral expression of mouse c-Myc V394D mutant

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc V394D (Myc Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    V394D (does not bind to Miz-1)

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTH726-CEN-RLuc/minCFLuc

    Plasmid

    #38210

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge