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pcDNA3 Rod Opsin

Plasmid

#109361

Purpose

Human rod opsin

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin (RHO Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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mNeon-Vamp2

Plasmid

#186619

Purpose

Encodes Vamp 2 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Vesicle Associated Membrane Protein 2 (VAMP2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Tags

V5

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_Δpvc10

Plasmid

#198448

Purpose

Produces PVCs containing truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_Δpvc10

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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GZnP2 in pcDNA3.1+

Plasmid

#161739

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Article

Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

Insert

GZnP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

SKL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP B55 alpha shRNA 3-1

Plasmid

#10679

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha shRNA (PPP2R2A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pBR-Lent-STAT5A-CA

Plasmid

#170662

Purpose

Lentiviral expression of constitutively active STAT5A.

Depositor

Alexander Ruthenburg

Article

Richter et al Elife. 2021 Jul 15;10. pii: 64960. doi:

Insert

STAT5A (STAT5A Human)

Use

Lentiviral

Mutation

H298R and S710F

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS017: ratiometric pHluorin (cytosolic)

Plasmid

#163052

Purpose

ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC7-syt1-GCaMP6f

Plasmid

#190896

Purpose

GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

Depositor

Tom Clandinin

Article

Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

Insert

syt1-GCaMP6f

Expression

Insect

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-8XHis-mCherry-Omp25

Plasmid

#236399

Purpose

Elutable MitoTag-AP system

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

8XHis-mCherry-Omp25

Use

Retroviral

Tags

8XHis-tag and mCherry

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1ER

Plasmid

#36325

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCN38-kimA-yfp

Plasmid

#227635

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

kimA-yfp

Expression

Bacterial

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-tagRFP

Plasmid

#185599

Purpose

Lentiviral construct for expression of tagRFP-T targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-tagRFP-T

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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S-HBsAg

Plasmid

#103013

Purpose

Expresses HBV Small envelope glycoprotein in mammalian cells

Depositor

Wang-Shick Ryu

Article

Wang et al PLoS Pathog. 2010 Jul 15;6(7):e1000986.

Insert

HBV Small envelope protein

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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mCherry-Nesprin-1αKASH

Plasmid

#187015

Purpose

Disruption of LINC Complex

Depositor

Jan Lammerding

Article

Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

Insert

Nesprin-1alphaKASH

Use

Lentiviral

Tags

mCherry

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMOS031: superecliptic pHluorin (endoplasmic reticulum)

Plasmid

#163051

Purpose

superecliptic pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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