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pYSD703

Plasmid

#253012

Purpose

Encodes Ideonella sakaiensis MHETase as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

MHETase

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_19 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-NLS-HbaUTR

Plasmid

#252015

Purpose

In vitro transcription of Cas9 only for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_17 - pLenti_EFS-Cas9-BARD1(32-117)-2A-BlastR

Plasmid

#252013

Purpose

Lentiviral mammalian expression of Cas9-BARD1(32-117) TruEditor

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BARD1(32-117)

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_16 - pLenti_EFS-Cas9-BLM(1-193)-2A-BlastR

Plasmid

#252012

Purpose

Lentiviral mammalian expression of Cas9-BLM(2-194) TruEditor

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BLM(2-194)

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-IRES-puro

Plasmid

#248148

Purpose

Lentiviral vector encoding human FCRL3

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-SlmiR482bTS-B/c

Plasmid

#234369

Purpose

Cloning syn-tasiRNA sequences in minimal SlmiR482bTS precursors for expression in tomato

Depositor

Alberto Carbonell

Article

Tomassi et al Plant Biotechnol J. 2025 Oct 14. doi: 10

Inserts

B/c
SlmiR482bTS

Expression

Plant

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR0-37a +SV40 base prom-GFP-IRES-AP

Plasmid

#225889

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR0-37a

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR3-17c +SV40 base prom-GFP-IRES-AP

Plasmid

#225890

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR3-17c

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 mR3-17d +SV40 base prom-GFP-IRES-AP

Plasmid

#225891

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

mR3-17d

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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Vsx2 Mini-enhancer +SV40 base prom-GFP-IRES-AP

Plasmid

#225893

Purpose

Enhancer reporter

Depositor

Michael Dyer

Article

Honnell et al Development. 2024 Jul 1;151(13):dev20243

Insert

VSX2 Mini-enhancer

Tags

EGFP

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-NAT

Plasmid

#221129

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC61-FAPoptim

Plasmid

#221126

Purpose

FAP-tagged marker for the ER (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-CFAPorig

Plasmid

#221092

Purpose

FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr-NFAPoptim

Plasmid

#221110

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-CUP1pr-CFAPoptim

Plasmid

#221111

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-NFAPoptim

Plasmid

#221088

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-CFAPoptim

Plasmid

#221089

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-NFAPorig

Plasmid

#221091

Purpose

FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS413-TEF1pr-NFAPoptim

Plasmid

#221093

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-CFAPoptim

Plasmid

#221094

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge