We narrowed to 17,812 results for: Met;

1. pET-Sac-Abeta(M1-40)

   Plasmid

   #71876

   Purpose

   Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-40) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSynSRE-Mut-T-Luc

   Plasmid

   #60490

   Purpose

   Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988

   Depositor

   Timothy Osborne

   Article

   Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.

   Insert

   HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
   

   Use

   Luciferase

   Tags

   Luciferase reporter

   Expression

   Mammalian

   Mutation

   Four point mutations (G->C or A->C) in the …

   Promoter

   Partial HMG-CoA synthase promoter (-324/-225) fus…

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. mRuby2-LaminA-C-18

   Plasmid

   #55901

   Purpose

   Localization: Nuclear Envelope, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3.1-nAChr-Alpha6-mCherry

   Plasmid

   #50480

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   mCherry fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBoBi-hTRPV4-C-GC6s

   Plasmid

   #154150

   Purpose

   To detect the calcium ion releases from TRPV4 channel

   Depositor

   Sheng-cai Lin

   Article

   Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

   Insert

   TRPV4 and GCamp6s (TRPV4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-DeAct-GS1

   Plasmid

   #89445

   Purpose

   Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-GS1 (GSN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning cytoplasmic Gsn amino acids 5…

   Promoter

   CMV

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG414GPD-dCas9-VPR

   Plasmid

   #63801

   Purpose

   pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   April 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRE-8XGTIIC-DsRED-DD

   Plasmid

   #115798

   Purpose

   lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

   Depositor

   Joan Massague

   Article

   Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

   Insert

   YAP responsive promoter (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   DsRED-DHFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. InsRA-eGFP

   Plasmid

   #79795

   Purpose

   Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   InsRA (INSR Human)
   

   Use

   Tags

   eGFP extracellular

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-Chronos-GFP

    Plasmid

    #99232

    Purpose

    AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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