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CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Neuroserpin oloop

Plasmid

#58260

Purpose

Retroviral vector for expressing mutated Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Mutant of neuroserpin lacking five residues from …

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
HcRed-pcw107

Plasmid

#64646

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Promoter

PGK

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only
pEF-TAZ-N-Flag (-4)

Plasmid

#40914

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin, and lacks the final fo…

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pRVH1-hygro shStx3

Plasmid

#40071

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Stx3 shRNA-1

Use

Retroviral

Promoter

H1

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-STAG2-K225insNM

Plasmid

#31974

Depositor

Todd Waldman

Article

Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

Insert

human STAG2 cDNA (STAG2 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

inserted amino acids Asn-Met before Lys225

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEF Itk P158A P159A myc

Plasmid

#20708

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk PR (Itk Mouse)

Tags

myc

Expression

Mammalian

Mutation

P158A P159A. Inactivates the proline-rich region.

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
pEN_TT PCSK5(sgRR,M452I)-V5

Plasmid

#232450

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5_M452I mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Mutation

a75g_t84c_g87c; M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5(sgRR,T288P)-V5

Plasmid

#232451

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5_T288P mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(T288P) (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Mutation

a75g_t84c_g87c; T288P

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK PCSK5(sgRR)-V5 hygro

Plasmid

#232452

Purpose

Lentiviral expression vector for an inducible sg09 resistant PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

a75g_t84c_g87c

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK PCSK5(sgRR,M452I)-V5 hygro

Plasmid

#232453

Purpose

Lentiviral expression vector for an inducible sg09 resistant PCSK5_M452I mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(M452I) (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

a75g_t84c_g87c; M452I

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK PCSK5(sgRR,T288P)-V5 hygro

Plasmid

#232454

Purpose

Lentiviral expression vector for an inducible sg09 resistant PCSK5_T288P mutant

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5(T288P) (PCSK5 Human)

Use

Lentiviral

Tags

V5

Mutation

a75g_t84c_g87c; T288P

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-CMV-DIO-MTS-roGFP-WPRE

Plasmid

#245375

Purpose

Cre-dependent expression of a green fluorescent protein biosensor to determine mitochondrial cellular reduction-oxidation (redox) states.

Depositor

D. James Surmeier

Article

Tubert et al Neurobiol Dis. 2023 Nov;188:106328. doi:

Insert

roGFP

Use

AAV

Tags

mitochondrial-targeting sequence of the S. pombe …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBit-CHIP-G132N

Plasmid

#236065

Purpose

SmBit-CHIP expression with G132N substitution

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

SmBit-CHIP-G132N (STUB1 Human)

Use

Luciferase

Tags

SmBit

Mutation

G132N

Promoter

HSV-TK

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBit-CHIP-K30A

Plasmid

#236066

Purpose

SmBit-CHIP expression with K30A substitution

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

SmBit-CHIP-K30A (STUB1 Human)

Use

Luciferase

Tags

SmBit

Mutation

K30A

Promoter

HSV-TK

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR223-ABL2(WT)

Plasmid

#215467

Purpose

Gateway entry vector with Arg wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Gateway entry vector

Mutation

Includes stop codon

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSUPER-Rac1(dog)

Plasmid

#228760

Purpose

A knockdown vector for dog Rac1.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A shRNA targeting the dog Rac1 gene (RAC1 canis lupus)

Expression

Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuideFE-mSG-Puro

Plasmid

#226522

Purpose

Cas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-Puro

Depositor

Neville Sanjana

Article

Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

Insert

hU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro

Use

CRISPR and Lentiviral

Tags

mStayGold

Expression

Mammalian

Promoter

U6,EF1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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Immunostealth

Plasmid

#239446

Purpose

Expresses murine NIS (mNIS) in mammalian cells for non-immunogenic in vivo imaging using SPECT or PET

Depositor

Scott Lyons

Article

Merrill et al Cell Stress. 2023 Aug 14;7(8):59-68. doi

Insert

sodium iodide symporter (Slc5a5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-flag-IARS2-Δ1-48

Plasmid

#232341

Purpose

Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340

Depositor

Quanfu Ma

Insert

IARS2 (IARS2 Human)

Expression

Mammalian

Mutation

Residues 1-48 (indicated as Mito-signal in UniPro…

Promoter

CMV

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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