We narrowed to 16,131 results for: form

1. pDEST-CMV GFP-LC3B G120

   Plasmid

   #123116

   Purpose

   Expresses EGFP-LC3B G120 in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 121-125. Stop codon after G12…

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CFP-GIV-CT FA

   Plasmid

   #69533

   Purpose

   Mammalian expression of The CFP-tagged GIV-CT-FA biosensors

   Depositor

   Pradipta Ghosh

   Article

   Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

   Insert

   GIV (CCDC88A Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   C terminal amino acids S1660-S1870 of human GIV w…

   Promoter

   CMV

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. human TLNRD1-F250D pET151

   Plasmid

   #159385

   Purpose

   Expresses human TLNRD1 F250D mutant in bacterial cells

   Depositor

   Ben Goult

   Article

   Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Changed Phenylalanine 250 to Aspartic Acid (F250D)

   Promoter

   T7

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCHKO_PDPR_exon_deletion_1

   Plasmid

   #155065

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLAP-BUBR1-dKARD

   Plasmid

   #114053

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1-dKARD (BUB1B Human)
   

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-CARMA2cl-FLAG

   Plasmid

   #97077

   Purpose

   Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

   Depositor

   Pasquale Vito

   Article

   Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

   Insert

   CARD14 isoform 2 (CARD14 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. CFP-GIV-CT WT

   Plasmid

   #69532

   Purpose

   Mammalian expression of The CFP-tagged GIV-CT biosensors

   Depositor

   Pradipta Ghosh

   Article

   Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

   Insert

   GIV (CCDC88A Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   C terminal amino acids S1660-S1870 of human GIV

   Promoter

   CMV

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pS6-C-hα2M-H6

   Plasmid

   #128497

   Purpose

   Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (F789-S1468) (A2M Human)
   

   Tags

   His tag (6x)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1 hSlp2-a del.SHD

   Plasmid

   #40050

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   hSlp2-a del.SHD (SYTL2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deletion of SHD domain (1-56)

   Promoter

   CMV promoter

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pLR5-CAG-NLS-tdiRFP670-2A-LBR

    Plasmid

    #82620

    Purpose

    Expresses NLS-tdiRFP-2A-LBR in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pαH-S-HRV3C/D614G

    Plasmid

    #164568

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-HRV3C-D614G variant)
    

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCHKO_Ptbp_ex8_3

    Plasmid

    #155083

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N3/hArf5(WT)-EGFP

    Plasmid

    #79534

    Purpose

    Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNAs[bTub+Sxl].1046B

    Plasmid

    #112692

    Purpose

    express two gRNA targeting bTub & Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGK-hMIGA2-DsRed

    Plasmid

    #192867

    Purpose

    Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Tags

    DsRed

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCHKO_SFRS7_exon_deletion_3

    Plasmid

    #155071

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCHKO_MDM4_exon_deletion_3

    Plasmid

    #155075

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1 hSlp4-a del.SHD

    Plasmid

    #40036

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a del.SHD (SYTL4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted SHD domain (1-143)

    Promoter

    CMV promoter

    Available Since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

19. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid

    #121813

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMX-IY LC3B

    Plasmid

    #89291

    Purpose

    For expression of the mammalian Atg8 protein LC3B without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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