We narrowed to 17,812 results for: Met;

1. pOTTC589 - pAAV c-fos Nuc-eYFP

   Plasmid

   #59133

   Purpose

   An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Nuclear-localized Enhanced Yellow Fluorescent Protein
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   mFos

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #86613

   Purpose

   Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC337 - pAAV EF1a DIO mCherry

   Plasmid

   #47636

   Purpose

   An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

   Plasmid

   #55648

   Purpose

   Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre off/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

   Plasmid

   #218529

   Purpose

   sgRNA targeting human EIF2AK1/HRI

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK1 (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pclbw-opa1(isoform 7DeltaS1)-myc

   Plasmid

   #62846

   Purpose

   Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   mmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)
   

   Use

   Retroviral

   Tags

   9xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   cmv-bactin

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mIFP-MAPTau-N-10

   Plasmid

   #56230

   Purpose

   Localization: Microtubules, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   MAPTau (MAPT Human)
   

   Use

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pRSET.GltI253-cpGFP.L1LV/L2NP

   Plasmid

   #41733

   Purpose

   a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Insert

   GltI based Glutamate Biosensor
   

   Use

   Glutamate biosensor

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

   Promoter

   T7

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. M_ST1n_VPR

   Plasmid

   #63799

   Purpose

   ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   ST1-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATP1A1_plasmid_donor_RD

    Plasmid

    #86551

    Purpose

    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 (ATP1A1 Human)
    

    Use

    CRISPR and TALEN; Co-selection via hdr using ouab…

    Tags

    Expression

    Mammalian

    Mutation

    Q118R, N129D

    Promoter

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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