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pJH3410

Plasmid

#179575

Purpose

Punc-129(DB)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans B-MN -specific expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Punc-129

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2890

Plasmid

#179561

Purpose

Plgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Plgc-55B

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2844

Plasmid

#179541

Purpose

Punc-25::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans D-MN and other neurals expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Punc-25

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2842

Plasmid

#179540

Purpose

Pacr-5::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans B-MN and other neurals expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pacr-5

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2843

Plasmid

#179389

Purpose

Punc-4::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A-MN and other neurals expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Punc-4

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-101-V5His6_D

Plasmid

#146121

Purpose

Insect Expression of DmEDC3_1-101

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-101 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_1-338-V5His6_D

Plasmid

#146122

Purpose

Insect Expression of DmEDC3_1-338

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-338 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_441-680_D

Plasmid

#146130

Purpose

Insect Expression of DmEDC3_441-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_441-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-D61A_D

Plasmid

#146131

Purpose

Insect Expression of DmEDC3-D61A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3-D61A (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-D61K_D

Plasmid

#146132

Purpose

Insect Expression of DmEDC3-D61K

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3-D61K (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-N44A_D

Plasmid

#146135

Purpose

Insect Expression of DmEDC3-N44A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3-N44A (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN S473A 513A

Plasmid

#175763

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

S473A S513A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-mRnf185

Plasmid

#175126

Purpose

Lentiviral expression of V5-tagged mouse Rnf185

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Rnf185 (Rnf185 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmem43-V5

Plasmid

#175111

Purpose

Lentiviral expression of mouse Tmem43-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Tmem43 (Tmem43 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR N52R (Shank3)

Plasmid

#175255

Purpose

SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with N52R mutation from SHANK3

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_L139*

Plasmid

#174400

Purpose

S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Mutation

L139* (Truncation after 139th amino acid in sarA)

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585-gp130

Plasmid

#174393

Purpose

Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA-IL6ST chimera

Tags

Flag

Expression

Mammalian

Mutation

140-177 of sarA replaced with homologous IL6ST se…

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#3

Plasmid

#163390

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSC51

Plasmid

#104825

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.12g075700, Glyma.11g145900

Use

CRISPR

Expression

Plant

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM L368G

Plasmid

#169978

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, L368G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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