We narrowed to 2,189 results for: levi

1. pCMV-PE2-P2A-GFP

   Plasmid

   #132776

   Purpose

   Prime editing in mammalian cells with co-translational GFP expression

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2-P2A-GFP
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PG13-luc (wt p53 binding sites)

   Plasmid

   #16442

   Purpose

   Luciferase reporter containing 13 copies of the p53-binding consensus sequence

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   p53-binding site (TP53 )
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189925

   Purpose

   AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SauriABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SauriCas9 D15A

   Promoter

   EFS

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn1-SIO-stGtACR2-FusionRed

   Plasmid

   #105677

   Purpose

   Expresses a soma-targeted GtACR2 in Cre-expressing neurons

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   stGtACR2
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CKIIa-stGtACR2-FusionRed

   Plasmid

   #105669

   Purpose

   Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV Retrograde and AAV1

   Insert

   stGtACR2
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

   Plasmid

   #135955

   Purpose

   Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   Prime editor v2 (PE2) N-terminal
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189922

   Purpose

   AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SaCas9 D10A

   Promoter

   EFS

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSynapsin-PE2-Cterminal-lenti

   Plasmid

   #135956

   Purpose

   Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   Prime editor v2 (PE2) C-terminal
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   MMLV RT(D200N, T306K, W313F, T330P, L603W)

   Promoter

   hSynapsin

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-PuroDTK.Neo

   Plasmid

   #72307

   Purpose

   PiggyBac Cassette contains puromycin and TK genes

   Depositor

   Yuet Wai Kan

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

   Insert

   Puromycin-DTK-neo
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   Feb. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Neo-PuroDTK

    Plasmid

    #84036

    Purpose

    PiggyBac cassette contains Neomycin, puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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