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We narrowed to 2,056 results for: ENDOG

Showing: 881 - 900 of 2056 results

  1. pX330_rActb sgRNA / hSpCas9

    Plasmid
    #172833
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172833-plasmid-map-sequence-id-340902

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  3. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid
    #172835
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172835-plasmid-map-sequence-id-341082

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  4. pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47445
    Purpose
    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NN_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47445-plasmid-map-sequence-id-72977

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  5. pX330_rSyn1 sgRNA / hSpCas9

    Plasmid
    #172840
    Purpose
    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172840-plasmid-map-sequence-id-340905

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  7. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #48253
    Purpose
    LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    CRY2PHR-NLS-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    48253-plasmid-map-sequence-id-70481

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  9. pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47451
    Purpose
    AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NG_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47451-plasmid-map-sequence-id-72980

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  10. pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47448
    Purpose
    AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NI_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    47448-plasmid-map-sequence-id-69440

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  11. pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47449
    Purpose
    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NN_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47449-plasmid-map-sequence-id-72979

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  12. pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47447
    Purpose
    AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NG_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47447-plasmid-map-sequence-id-72978

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  15. pAC1-pCR8-dCas9VP48

    Plasmid
    #48214
    Purpose
    dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP48 activation domain
    Use
    CRISPR; Gateway donor
    Tags
    HA Tag and VP48
    Mutation
    D10A;H840A
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48214-plasmid-map-sequence-id-69493

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  17. pGS1212

    Plasmid
    #63785
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eGFP-Strep-tag II
    Promoter
    none
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63785-plasmid-map-sequence-id-106471

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Showing: 881 - 900 of 2056 results