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Addgene

We narrowed to 161,813 results for: addgene

Showing: 881 - 900 of 161813 results
  1. cTRE-PtenWT

    Plasmid
    #135663
    Purpose
    Introduce Dox-inducible (TRE promoter) wildtype Pten cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Insert
    Pten (Pten Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pTF280 (pFA6a-PSc-6xGly-2xStrep-HphMX)

    Plasmid
    #44076
    Depositor
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
  3. pGRHL2.1.0-gDNA

    Plasmid
    #175863
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Insert
    GRHL2 (GRHL2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. 5E16 (PfPKG, PF14_0346)

    Plasmid
    #79518
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    PF14_0346
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. TMPRSS2 g4 + Cas9

    Plasmid
    #153018
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. WNK3 (WNK3B-c016)

    Plasmid
    #110252
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    WNK3 (WNK3 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. WNK1 (WNK1A-c011)

    Plasmid
    #98232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    WNK1A (WNK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pPyCAG-Nanog(L122A)-IP

    Plasmid
    #62992
    Purpose
    Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Insert
    Mouse Nanog L122A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Leucine 122 (123 AA in Mouse Protein) to …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. pZBTB12.1.0-gDNA

    Plasmid
    #113769
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB12
    Depositor
    Insert
    ZBTB12 (ZBTB12 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pZBTB17.1.0-gDNA

    Plasmid
    #112483
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB17
    Depositor
    Insert
    ZBTB17 (ZBTB17 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pSPDEF.1.0-gDNA

    Plasmid
    #113796
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SPDEF
    Depositor
    Insert
    SPDEF (SPDEF Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)

    Plasmid
    #44087
    Depositor
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
  14. pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)

    Plasmid
    #44078
    Depositor
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  15. pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)

    Plasmid
    #44088
    Depositor
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
  16. pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)

    Plasmid
    #44075
    Depositor
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    12xHis tag, 6xGly linker, ADH1 transcription term…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
  17. pMLX.1.0-gDNA

    Plasmid
    #112466
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MLX
    Depositor
    Insert
    MLX (MLX Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  18. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. USP37

    Plasmid
    #36898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    USP37 (USP37 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 4-125 only
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  20. MLH1

    Plasmid
    #28120
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Use
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-347.
    Promoter
    Available since
    March 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 881 - 900 of 161813 results