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Plasmid#59280PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.DepositorInsertIntergenic 2kb upstream krt18
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-INT2
Plasmid#59287PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the keratin type II cluster.DepositorInsertkrt80-Intron 4
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter cel-mir-235
Plasmid#46660DepositorInsertcel-mir-235
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter cel-mir-124
Plasmid#46659DepositorInsertcel-mir-124
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter dme-mir-279
Plasmid#46668DepositorInsertdme-mir-279
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter dme-mir-92a
Plasmid#46666DepositorInsertdme-mir-92a
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt344B
Plasmid#34602DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-344 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S-Mt206A
Plasmid#34599DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationThe KLF4 K5S region was mutated at the miR-206 bi…PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA
Plasmid#84468PurposepAAV-CRAM-tdTomatoDepositorInserttdTomato
UseAAVTagsExpressionMutationT230I (please see depositor comments below)PromoterTREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
Plasmid#221356PurposeMammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa; to be used with Anti-Strep [IPI-C23.21] heavy chain (Plasmid 221355) to make the antibody.DepositorInsertAnti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
Plasmid#141385PurposeLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-E (E SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1
Plasmid#122084PurposeExpress M.EcoGII fused to TRF1 with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-TERF1
UseRetroviralTagsV5 tag on Nter of TERF1ExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
Plasmid#84474PurposepAAV-RAM-NLS-mKate2DepositorInsertmKate2
UseAAVTagsNuclear localization signal of SV40 large T antig…ExpressionMutationPromoterTREAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
Plasmid#141386PurposeLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-M (M SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
Plasmid#141391PurposeLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-N (N SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA
Plasmid#171383PurposeExpression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized minidCas13X.1-REPAIRv2
UseTagsHAExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR
Plasmid#171382PurposeExpression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized dCas13X.1-REPAIRv2
UseTagsHAExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI
Plasmid#115211PurposeExpresses PsmCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPsmCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0052-REPAIR non-targeting guide clone into pC0043
Plasmid#103868PurposeNon-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.DepositorInsertnon-targeting guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172503PurposeEncodes the DisCas7-11 protein only for bacterial recombinant protein expression for purificationDepositorInsertDisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI
Plasmid#115213PurposeExpresses RanCas13b from a SUMO-tagged bacterial expression constructDepositorInsertRanCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI
Plasmid#115218PurposeExpresses PguCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPguCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV(cmv)-NEO-FSF-tdTomato Cre reporter
Plasmid#160551Purposelabeling hypoxic cells, Cre reporterDepositorInserttdTomato
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-HA-KORD-IRES-mCitrine-WPREpA
Plasmid#154871PurposeHuman synapsin-1 promoter; Flp-dependent KORD-IRES-mCitrineDepositorInsertKORD-IRES-mCitrine
UseAAV; Flp-frtTagsHAExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172505PurposeEncodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purificationDepositorInsertCjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry
Plasmid#172637PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPro287AlaPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0051-W85X REPAIR targeting guide cloned into pC0043
Plasmid#103867PurposeGuide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038DepositorInsertW85X targeting guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI
Plasmid#115219PurposeExpresses PspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPspCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final
Plasmid#107009PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI
Plasmid#115209PurposeExpresses PbuCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPbuCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only