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Plasmid#127368PurposeBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
UseTagsGS linker, NLS, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-Turbo-NES-YFP
Plasmid#127366PurposeBinary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
UseTagsGS linker, NES, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-miniTurbo-NES-YFP
Plasmid#127369PurposeBinary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plantsDepositorInsertminiTurbo (BirA mutant)
UseTagsGS linker, NES, V5, and YFPExpressionPlantMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMAL-PIAL2M-sim1
Plasmid#67908PurposeInducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.DepositorInsertPIAL2 (AT5G41580 Mustard Weed)
UseTagsMBPExpressionBacterialMutationE281-A496; VEDL 425-428 AAAPromoterP-lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-RGG-SmBit-RGG
Plasmid#176644PurposeSmBit fused to LAF-1 RGG domain with N-terminal MBP tagDepositorInsertMBP-RGG-SmBit-RGG
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-RGG-LgBit-RGG
Plasmid#176643PurposeLgBit fused to LAF-1 RGG domain with N-terminal MBP tagDepositorInsertMBP-RGG-LgBit-RGG
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
UseTagsExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
YUC4:RUBY
Plasmid#160907PurposeRUBY under the control of YUC4 promoter/tissue specific marker for gene expressionDepositorInsertRUBY and YUC4 promoter
UseTagsExpressionPlantMutationPromoterYUC4Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB106-MTS-Citrine
Plasmid#79135PurposeBinary vectors for expression of Citrine fluorescent protein with mitochondria localization signal in plantsDepositorInsertSD3(1-50)
UseTagsCitrineExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P1
Plasmid#194343PurposesgRNA position entry clone 1DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHBAD42
Plasmid#124359PurposeArabinose-inducible vector encoding the C-terminal gp41-1 split fragment fused to GB1DepositorInsertgp41-1 C-intein
UseTagsGB1 added during cloningExpressionBacterialMutationPromoterParaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2:GFP
Plasmid#218565PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1:GFP
Plasmid#218564PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Mouse 3' Ubf AID GFP PuroR
Plasmid#127901PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf GeneDepositorInsertUbf (Ubtf Mustard Weed, Mouse)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationInserting GFP AID 2A Puro into mouse 3' UbfPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.E222
Plasmid#138248PurposeThree input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promotersDepositorInsertsaraC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_AtCor47 (pBS0784)
Plasmid#185254PurposeFor the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertDHN_AtCor47
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AD-uvr8at
Plasmid#44971DepositorInsertAD-UVR8 (UVR8 Mustard Weed)
UseTagsNF-κB activation domainExpressionMammalianMutationdeleted Methionine 1PromoterpCMVAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMM660
Plasmid#68889PurposeArabinogalactan-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only