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pLenti-dSaCas9-KRAB-blast

Plasmid

#191654

Purpose

Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

dSaCas9-KRAB

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-HCF-1rep1E10A

Plasmid

#84607

Purpose

Plasmid contains HCF-1rep1E10A with GST-tag

Depositor

Winship Herr

Article

Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

Insert

HCF-1rep1E10A (HCFC1 Human)

Use

Tags

GST and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-HCF-1rep1wt

Plasmid

#84606

Purpose

Plasmid contains HCF-1rep1wt with GST-tag

Depositor

Winship Herr

Article

Capotosti et al Cell. 2011 Feb 4;144(3):376-88. doi: 10.

Insert

HCF-1rep1wt (HCFC1 Human)

Use

Tags

GST-TEV and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pT/Caggs-NRAS G12V-IRES-ASK1

Plasmid

#221074

Purpose

Expresses NRAS G12V and ASK1 in mammalian cells

Depositor

Hidenori Ichijo

Article

Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

Inserts

NRAS (NRAS Human)
ASK1

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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HA, EGFP Spastin M87 with exon 4, N386C

Plasmid

#167543

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4, with N386C mutation.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Use

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

N386C

Promoter

CMV

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-RNF114-RINGmut

Plasmid

#69922

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

Expression

Mammalian

Mutation

C29A, C32A

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pACEBac1-beta-actin

Plasmid

#178076

Purpose

Insect cell expression of beta-actin

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

beta-actin (ACTB Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-MIDAS

Plasmid

#171604

Purpose

Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)

Use

Tags

His6 tag and SNAP tag

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-RNF114-UIMmut

Plasmid

#69923

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

Expression

Mammalian

Mutation

S224P

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pYPQ269A2

Plasmid

#164714

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265

Plasmid

#164712

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

Plasmid

#129105

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

Depositor

Eleftherios Terry Papoutsakis

Article

Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

Insert

BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Zebrafish-gRNA-0001

Plasmid

#42241

Purpose

gRNA targeted to zebrafish gene apoea

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-apoea (apoea Zebrafish)

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

Plasmid

#205603

Purpose

Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinWT-RNAi-resistant-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

Plasmid

#205604

Purpose

Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinΔGTP-RNAi-resistant-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A, N229A

Promoter

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Sa-gRNA-puro

Plasmid

#191652

Purpose

Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

Insert

S. aureus gRNAscr

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YE2

Plasmid

#154146

Purpose

Expresses dLbCpf1-BE-YE2(dLbCas12a-BE-YE2) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE2 (dCas12a-BE-YE2)

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R132E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-YEE

Plasmid

#154148

Purpose

Expresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YEE (dCas12a-BE-YEE)

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…

Promoter

CMV

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:S495A

Plasmid

#98151

Purpose

Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:S495A

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed serine 495 to alanine

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:W603A

Plasmid

#98153

Purpose

Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:W603A

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tryptophan 603 to alanine

Promoter

T7

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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