Addgene

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pNR230

Plasmid

#79546

Purpose

BxbI attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pNR276

Plasmid

#79577

Purpose

TP901 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR239

Plasmid

#79576

Purpose

A118 attB-attP. GFP will express if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pNR280

Plasmid

#79579

Purpose

A118 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pNR279

Plasmid

#79578

Purpose

BxbI attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR287

Plasmid

#79580

Purpose

TP901 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEPOZ0CM0137

Plasmid

#197535

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

C-terminal glucocorticoid receptor, GR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEPOZ1KN0145

Plasmid

#197552

Purpose

Level 1 gene expression cassette

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

2xCaMV35s::TMV::AtANAC032:GR::35sT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEPOZ1KN0144

Plasmid

#197551

Purpose

Level 1 gene expression cassette

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

2xCaMV35s::TMV::AtDREB26:GR::35sT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOZ1KN0142

Plasmid

#197549

Purpose

Level 1 gene expression cassette

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

2xCaMV35s::TMV::AtNLP6:GR::35sT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOZ1KN0143

Plasmid

#197550

Purpose

Level 1 gene expression cassette

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

2xCaMV35s::TMV::AtNLP7:GR::35sT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOZ1KN0147

Plasmid

#197554

Purpose

Level 1 gene expression cassette

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

2xCaMV35s::TMV::AtARF18:GR::35sT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOZ1KN0148

Plasmid

#197555

Purpose

Level 1 gene expression cassette

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

2xCaMV35s::TMV::AtARF9:GR::35sT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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KRD22_HBA1(HBA1reg-HBB-longHAs)

Plasmid

#232403

Purpose

AAV production plasmid for HBA1 long HAs vector from Figs. 3-6 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank full HBA1-2A-YFP cassette. HAs are ~900bp

Depositor

Kyle Cromer, Matthew Porteus

Article

Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

Inserts

Hemoglobin Subunit Alpha 1 (HBA1 Human)
Human Hemoglobin Subunit Beta (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-GR11

Plasmid

#89105

Purpose

mammalian expression of full-length human glucocorticoid receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Kupfer et al J Biol Chem. 1993 Aug 15;268(23):17519-2

Insert

human glucocorticoid receptor (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pNR188

Plasmid

#79543

Purpose

Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR284

Plasmid

#79550

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge