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pPD304 VPR-ZF43

Plasmid

#138840

Purpose

Constitutive expression of VPR-ZF43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mStayGold[E138D]

Plasmid

#221049

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mStayGold[E138D]

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_SUMO_AcCas12n

Plasmid

#203808

Purpose

pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

SUMO_AcCas12n

Use

CRISPR

Promoter

T7

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1171 FIREmate-ECFP-Giantin

Plasmid

#205166

Purpose

Expresses FIREmate-ECFP-Giantin in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Giantin

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRCA918 - TFORF Empty Vector Puro-P2A-BFP (pRCA892 backbone)

Plasmid

#238183

Purpose

Lentiviral empty vector

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Expression

Bacterial

Promoter

Ec-TTL-P112 and BBa_J23116

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

Plasmid

#84251

Purpose

Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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His SUMO HNRNPA1 dHexa

Plasmid

#197013

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-MapTau

Plasmid

#137808

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

MapTau

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQAT09

Plasmid

#223381

Purpose

T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-SpRY-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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