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pRDB_131

Plasmid

#216095

Purpose

Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD97, CD4, CD26, CD274 guides

Use

CRISPR and Lentiviral; Positive controls

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk2a

Plasmid

#208983

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Publication

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk2a (kcnk2a Zebrafish)

Use

Cloning

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pADH2(D)_P1(SBE214)

Plasmid

#195015

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(B) pADH2 (R)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_EF-act1_full

Plasmid

#202172

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 full terminator

Use

Synthetic Biology

Mutation

None

Available since

Sept. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-act1_tr

Plasmid

#202171

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 truncated terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-PT2A

Plasmid

#202138

Purpose

Level 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructs

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Dual 2A peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-mito_sig_pep

Plasmid

#202134

Purpose

Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Mitochondria signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-sec_sig_pep

Plasmid

#202133

Purpose

Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Secretion signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-chl_sig_pep

Plasmid

#202131

Purpose

Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Chloroplast signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pJL1-MBP-DQNAT

Plasmid

#198215

Purpose

pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

MBP_DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrSmg6_F

Plasmid

#146279

Purpose

Bacterial Expression of DrSmg6

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg6 (LOC100334020 Zebrafish)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrUPF2_F

Plasmid

#146282

Purpose

Bacterial Expression of DrUPF2

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF2 (upf2 Zebrafish)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY

Plasmid

#193555

Purpose

Expresses mEGFP-ShadowY in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

ShadowY

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pME-p3E-GLRX-roGFP2

Plasmid

#194288

Purpose

Multisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQF

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

Gateway Cloning

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-P662K-GFP

Plasmid

#185591

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

P662K

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-mCherry-LOV

Plasmid

#185585

Purpose

for photoconversion

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry-LOV

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pE5773

Plasmid

#170780

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Publication

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA-HA

Plasmid

#186374

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-HA-RfA

Plasmid

#186373

Purpose

Destination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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