Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pSDS088

Plasmid

#250583

Purpose

in vivo Inverter Plasmid

Depositor

Timothy Whitehead

Inserts

HOT5(mandi) (PYR1 Mustard Weed)
LgBiT-HOT5(Constitutive) (PYR1 Mustard Weed)
SmBiT-ΔNHAB1 (HAB1 Mustard Weed)

Use

Synthetic Biology

Tags

LgBiT and SmBiT

Expression

Bacterial

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pKR7:H2A1p-(linker2)-AFP BD-AS(50bp)2mm(18-19)-(linker3)-(Canonical 3'SE V2 mutated PPT)-mK2-Ex2-(linker4)-pA

Plasmid

#251267

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Article

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1717_X

Plasmid

#147982

Purpose

Insect Expression of DmNot1_1-1717

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717 (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1941-2505-dsRNAres_W

Plasmid

#147858

Purpose

Insect Expression of DmNot1_1941-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1941-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182_635-1384_W

Plasmid

#147831

Purpose

Insect Expression of DmGW182_635-1384

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmGW182_635-1384 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-812_W

Plasmid

#147844

Purpose

Insect Expression of DmNot1_1-812

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-812 (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147846

Purpose

Insect Expression of DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-del1967-2251-dsRNAres_U

Plasmid

#147711

Purpose

Insect Expression of DmNot1-del1967-2251-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot1-del1967-2251-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNOT1_1-1083_R

Plasmid

#147441

Purpose

Bacterial Expression of DmNot1_1-1083

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1083 (Not1 Fly)

Expression

Bacterial

Mutation

4 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_416-1148_R

Plasmid

#147418

Purpose

Insect Expression of DmNot1_416-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_416-1148 (Not1 Fly)

Expression

Insect

Mutation

3 silent and two non silent N605S, K759M mutation…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_168-552-D412AN414A_R

Plasmid

#147427

Purpose

Insect Expression of DmCCR4_168-552-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552-D412AN414A (twin Fly)

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-K1277A-dsRNAres_R

Plasmid

#147359

Purpose

Insect Expression of DmNot1-K1277A-dsRNAres

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

DmNot1-K1277A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-Q1271A-dsRNAres_R

Plasmid

#147360

Purpose

Insect Expression of DmNot1-Q1271A-dsRNAres

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

DmNot1-Q1271A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-R1267A-dsRNAres_R

Plasmid

#147361

Purpose

Insect Expression of DmNot1-R1267A-dsRNAres

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

DmNot1-R1267A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1710-2505_J

Plasmid

#146701

Purpose

Insect Expression of DmNot1_1710-2505

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1710-2505 (Not1 Fly)

Expression

Insect

Mutation

one non silent mutation (A1747T) and one silent m…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1-1148_I

Plasmid

#146613

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Expression

Insect

Mutation

four silent and two non silent N605S, K759M mutat…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmTral_1-84_C

Plasmid

#146082

Purpose

Bacterial Expression of DmTral_1-84

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_1-84 (tral Fly)

Expression

Bacterial

Mutation

one silent mutation A198C compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-Q281AH284A_E

Plasmid

#146204

Purpose

Insect Expression of DmMe31B-Q281AH284A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-Q281AH284A (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-T288AK292A_E

Plasmid

#146205

Purpose

Insect Expression of DmMe31B-T288AK292A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

DmMe31B-T288AK292A (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and two non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC

Plasmid

#148404

Purpose

Insect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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