We narrowed to 18,897 results for: Met;

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Salsa-BC2-P1A2

Plasmid

#202843

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC2

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pXWJ109As-gfp

Plasmid

#123143

Purpose

An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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Dendra2-EB3-7

Plasmid

#57715

Purpose

Localization: MT End Binding Protein, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

EB3 (MAPRE3 Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pVH72-Tier1-PhCMV-SEAP

Plasmid

#169560

Purpose

Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven human secreted placental alkaline phosphatase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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Salsa-BC6-P1A6

Plasmid

#202847

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC6

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::NeoR-bGHpA

Plasmid

#62438

Purpose

MXS_chaining vector with PGK::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-CmCHI

Plasmid

#73393

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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fADL-1e-flag-linker-p3

Plasmid

#139441

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, flag-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoNanobody IRES EGFP

Plasmid

#136621

Purpose

Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST(frt)(ATG_V5-APEX2_noSTOP)

Plasmid

#168207

Purpose

Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Type

Empty backbone

Use

Unspecified; Pdest gateway

Tags

V5 APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-CathespinB-6

Plasmid

#57354

Purpose

Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CathespinB (Ctsb Rat)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pACYCDuet_D27NDHFR_WTTS

Plasmid

#91244

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31YG121VDHFR_WTTS

Plasmid

#91229

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_G121VDHFR_WTTS

Plasmid

#91233

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

G121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pPD385 VPR-ZF43(RAAA)

Plasmid

#138824

Purpose

Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(RAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-WDR5 shRNA 2

Plasmid

#59975

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pSB1K3_pBen-sfGFP_J23101-BenR

Plasmid

#128125

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP and BenR

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBen and J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pVH232-Tier1-PmPGK1-mRuby2

Plasmid

#169523

Purpose

Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric red fluorescent protein mRuby2

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP670nano3

Plasmid

#184672

Purpose

G protein alpha-subunit internally tagged with miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

G protein alpha-s-miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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fADL-1e-HA-linker-p3

Plasmid

#139440

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, HA-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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