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pLenti-CMV-Blast-DEST N-HPV101 E7

Plasmid

#253677

Purpose

Expresses N-Flag/HA HPV101 E7 in lentiviral background

Depositor

Karl Munger

Article

Gelbard et al Tumour Virus Res. 2025 Jun;19:200300. do

Insert

HPV101 E7

Use

Lentiviral

Tags

Flag/HA

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DEST C-HPV101 E7

Plasmid

#253676

Purpose

Expresses C-Flag/HA HPV101 E7 in lentiviral background

Depositor

Karl Munger

Article

Gelbard et al Tumour Virus Res. 2025 Jun;19:200300. do

Insert

HPV101 E7

Use

Lentiviral

Tags

Flag/HA

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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LXSN HPV101 E7

Plasmid

#253679

Purpose

Expresses HPV101 E7 in retroviral background

Depositor

Karl Munger

Article

Gelbard et al Tumour Virus Res. 2025 Jun;19:200300. do

Insert

HPV101 E7

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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p35C-gRNA-ccdB

Plasmid

#246792

Purpose

Express gRNA in plant cells (protoplast or callus) for Cas9-mediated editing

Depositor

Caixia Gao

Article

Li et al Nat Biotechnol. 2026 Jun 5. doi: 10.1038

Insert

ccdB

Use

CRISPR

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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pECFP-C1-PHF13

Plasmid

#246107

Purpose

Expression of human PHF13 fused to ECFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Tags

ECFP

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEYFP-C1-PHF13

Plasmid

#246098

Purpose

Expression of human PHF13 fused to mEYFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Tags

mEYFP

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PHF13

Plasmid

#246093

Purpose

Expression of human PHF13 fused to EGFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Tags

EGF

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV4-PHF13

Plasmid

#246094

Purpose

Expression of human PHF13 with Flag-tag in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-d7F

Plasmid

#245784

Purpose

Expression of mutated bovine arrestin-3 (deleted residues 175, 177, 178, 182-185) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

deleted residues 175, 177, 178, 182-185

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)

Plasmid

#239260

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

Plasmid

#239261

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RPPH1 (pAVA3585)

Plasmid

#239262

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPPH1

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPPH1

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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POC1553

Plasmid

#238470

Purpose

pMOBC360_VL: Vector Left (VL) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_MBP-VidN

Plasmid

#241013

Purpose

VidN construct that can be bacterially expressed, used to demonstrate intein splicing reaction.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

MBP-VidN

Tags

MBP-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb827

Plasmid

#217500

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb847

Plasmid

#217502

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb867

Plasmid

#217503

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb887

Plasmid

#217504

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVAb897

Plasmid

#217505

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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