We narrowed to 19,676 results for: Cre

1. TPST2-EGFP

   Plasmid

   #66618

   Purpose

   Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)

   Depositor

   David Stephens

   Article

   Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

   Insert

   TPST2 (TPST2 Human)
   

   Expression

   Mammalian

   Mutation

   Insert contains T370A relative to NM_001008566.1

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. EF1a_SPI1_P2A_Hygro_Barcode

   Plasmid

   #120482

   Purpose

   Barcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-EGFP

   Plasmid

   #209057

   Purpose

   Gateway-compatible entry vector, with insert of the EGFP gene

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PBE-NG

   Plasmid

   #229045

   Purpose

   Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.

   Depositor

   Huawei Zhang

   Article

   Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. p23-NES-PguCas13b-msfGFP-NES-Flag

   Plasmid

   #165072

   Purpose

   overexpression of PguCas13b in human cells

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2puro-Ptgs2_B6

   Plasmid

   #170300

   Purpose

   A knock-out vector for the mouse Ptgs2 in C57BL6

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse tyrosinase gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

   Plasmid

   #138145

   Purpose

   Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   NLS

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mKate2-P2A-APEX2-TAPP1-PH

   Plasmid

   #67662

   Purpose

   Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-TAPP1-PH
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. NPY-pHTomato

   Plasmid

   #83501

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Tags

   pHTomato

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

    Plasmid

    #52889

    Purpose

    "CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

    Depositor

    Iswar Hariharan

    Article

    Bosch et al Development. 2015 Feb 1;142(3):597-606.

    Insert

    Gal4 (GAL4 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)

    Plasmid

    #139844

    Purpose

    Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    AMPK-Kinase-dead (Prkaa2 Rat)
    

    Use

    Lentiviral

    Tags

    GST

    Mutation

    K47R

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-neo-Flag-DHHC5

    Plasmid

    #85812

    Purpose

    To rescue the expression of DHHC5 in the knockdown cell lines

    Depositor

    Sandra Hofmann

    Article

    Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

    Insert

    ZDHHC5 (Zdhhc5 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDuet TDP-43 M337V

    Plasmid

    #27464

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Expression

    Bacterial

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

14. Ii-Str_TNF-SBP-EGFP

    Plasmid

    #65280

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTag-BFP-C-h-Rab5a-c-Myc

    Plasmid

    #79801

    Purpose

    Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab5a (RAB5A Human)
    

    Tags

    TagBFP and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. LentiCRISPRv2hyg-Ptgs1

    Plasmid

    #170299

    Purpose

    A knock-out vector for the mouse Ptgs1

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Ptgs1 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH_Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65267

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. KHBD00188

    Plasmid

    #34340

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1034 (bcd Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. NPY1R-DuET

    Plasmid

    #213354

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NPY1R (NPY1R Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCold TDP43 M337V

    Plasmid

    #27453

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only