We narrowed to 16,006 results for: grna
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Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i5 sgRNA / hSpCas9
Plasmid#172829PurposeMammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i3 sgRNA / hSpCas9
Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7gRNA-ttn.1_C3
Plasmid#140866PurposesgRNA synthesis vector for ttn.1_C3 (zebrafish titin .1).DepositorInsertzebrafish ttn.1_C3 sgRNA for in vitro transcription (ttn.1 Zebrafish, Synthetic)
UseIn vitro transcription of sgrnasAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_FANCF-mcherry
Plasmid#159786PurposeS. pyogenes sgRNA collocated with pegRNA targeting human FANCF geneDepositorInsertspacer of sgRNA targeting FANCF gene (FANCF Human)
ExpressionMammalianAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 S3070F
Plasmid#139325PurposePlasmid expressing a sgRNA to introduce BRCA2 S3070F using base editingDepositorInsertsgRNA to insert BRCA2 S3070F using base editing
ExpressionMammalianPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA1 S1363L
Plasmid#139329PurposePlasmid expressing a sgRNA to introduce BRCA1 S1363L using base editingDepositorInsertsgRNA to insert BRCA1 S1363L using base editing
ExpressionMammalianPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA1 E1754K
Plasmid#139330PurposePlasmid expressing a sgRNA to introduce BRCA1 E1754K using base editingDepositorInsertsgRNA to insert BRCA1 E1754K using base editing
ExpressionMammalianPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA FANCD2 Q223Stop
Plasmid#139332PurposePlasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editingDepositorInsertsgRNA to insert FANCD2 Q223Stop using base editing
ExpressionMammalianPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGRNA-LbCpf1-SpCas9
Plasmid#119673PurposeEmpty vector for cloning LbCpf1-SpCas9 fgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFGRNA-AsCpf1-SpCas9
Plasmid#119674PurposeEmpty vector for cloning AsCpf1-SpCas9 fgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVC-Ds-DrU6a:scrambled1-sgRNA-Ds
Plasmid#119070PurposeConstruct with scrambled-1 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled1-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled2-sgRNA-Ds
Plasmid#119071PurposeConstruct with scrambled-2 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled2-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled3-sgRNA-Ds
Plasmid#119072PurposeConstruct with scrambled-3 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled3-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:scrambled4-sgRNA-Ds
Plasmid#119073PurposeConstruct with scrambled-4 sgRNA sequence for microinjections in zebrafishDepositorInsertU6a:scrambled4-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Puro_hs_NC_chr1
Plasmid#214688PurposeLentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)DepositorInsertdgRNA_Chr1
UseCRISPR and LentiviralMutationPuromycin resistance cassette has silent mutation…Available sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti ccdB Dang sgRNA Hyg sfGFP
Plasmid#235047PurposeLentiviral sgRNA expression with optimized sgRNA scaffold vector with hygromycin and superfolder GFP. Also contains ccdB cassette for library cloning.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceApril 30, 2026AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lentivirus ABE8e-V106W with non-targeting sgRNA
Plasmid#247668PurposeLentivirus genome encoding ABE8eV106W editor and Sp sgRNA cassette non-targeting controlDepositorInsertABE8e-V106W and non-targeting sgRNA
UseLentiviralExpressionMammalianPromoterEFSAvailable sinceApril 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-BbsI-CMV-EGFP cloning vector
Plasmid#239464PurposesgRNA cloning vector with EGFP expression cassette. An sgRNA spacer sequence can be cloned into BbsI sites. A CMV-EGFP expression cassette is included as a reporter of transfection efficiency.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6 and CMVAvailable sinceDec. 8, 2025AvailabilityAcademic Institutions and Nonprofits only