We narrowed to 19,281 results for: Cre

1. pCMV_APOBEC1-UdgX-nCas9

   Plasmid

   #163531

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. phFluc-WT

   Plasmid

   #177714

   Purpose

   Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

   Depositor

   Erik Böttger

   Article

   Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

   Insert

   hFluc WT
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LifeAct-mAvicFP1

   Plasmid

   #129512

   Purpose

   Expresses LifeAct-mAvicFP1 in mammalian cells

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   LifeAct-mAvicFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mAvicFP1 is AvicFP1 F64L + A206K

   Promoter

   CMV

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_NEUROG1_P2A_Hygro_Barcode

   Plasmid

   #120467

   Purpose

   Barcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NEUROG1 (NEUROG1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sema4a-Fc-His

   Plasmid

   #72154

   Purpose

   Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema4a (Sema4a Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDECKO_mCherry_CD19.4

   Plasmid

   #140101

   Purpose

   CRISPR-Cas9 Knock down

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXPR_003 sgMYC guide 2

   Plasmid

   #193597

   Purpose

   cMYC knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgMYC guide 2 (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pECE-M2-PAK2 S20A

   Plasmid

   #31681

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   p21-activated protein kinase 2 S20A (PAK2 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   changed Serine 20 to Alanine

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.10-VEGFprom(-950 to -700)

   Plasmid

   #66133

   Purpose

   Luciferase-based reporter for VEGF promoter region (-950 to -700 bp)

   Depositor

   David Mu

   Article

   Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

   Insert

   Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   VEGF -950 to -700

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAf-CRISPR-phoA

    Plasmid

    #207991

    Purpose

    CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    phoA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus flavus U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. DCT.VV.Orf40

    Plasmid

    #12728

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf40
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pAAV_12xTEAD_luc2

    Plasmid

    #173004

    Purpose

    firefly luciferase reporter vector with 12x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    12xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCold TDP43 G294A

    Plasmid

    #27452

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

14. GFP-SP110C

    Plasmid

    #65393

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP110C
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCMV_EE-nCas9

    Plasmid

    #163541

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    EE-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA1 Flag ATXN1L

    Plasmid

    #33242

    Depositor

    Huda Zoghbi

    Article

    Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

18. plKO.1-DNAJC5-ShRNA

    Plasmid

    #205729

    Purpose

    Knockdown of DNAJC5

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    shRNA targeting DNAJC5 (DNAJC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sema6d.1-AP-His

    Plasmid

    #72042

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.1 (Sema6d Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. KK501: pMVP (L3-L2) FKBP DD + polyA

    Plasmid

    #121789

    Purpose

    pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FKBP Degron Domain + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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