We narrowed to 9,921 results for: pET

Showing: 9121 - 9140 of 9921 results

Results per page:

Sort results by:

RhoC gRNA #3

Plasmid

#198726

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #3

Depositor

Takeshi Imai

Publication

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CCM2(413-438)_pHisSUMO(K0)

Plasmid

#224065

Purpose

Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site

Depositor

Ben Goult

Publication

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

ccm2 (CCM2 Human)

Tags

6xHIS; SUMO(K0)

Expression

Bacterial

Promoter

T7

Available since

Aug. 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
H6Gb1-CREB(1-100)

Plasmid

#217978

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Publication

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
H6Gb1-CREB(1-280)

Plasmid

#217981

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Publication

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pInducer20-iCreb5(HA)

Plasmid

#195023

Purpose

HA-tagged Creb5 in pInducer20 lentivirus destination vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GL4.10 E2E1- Prg4 promoter-luciferase

Plasmid

#195035

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)

Use

Luciferase

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

Plasmid

#195037

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)

Use

Luciferase

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hPRL1_1-169

Plasmid

#209792

Purpose

Bacterial expression of human PRL-1 without farnesylation motif

Depositor

Kalle Gehring

Publication

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-1 (PTP4A1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2753

Plasmid

#195637

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

Depositor

Gira Bhabha, Damian Ekiert

Publication

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; I241N)

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2754

Plasmid

#195638

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

Depositor

Gira Bhabha, Damian Ekiert

Publication

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; L245N)

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2755

Plasmid

#195639

Purpose

Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

Depositor

Gira Bhabha, Damian Ekiert

Publication

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP-MlaA

Expression

Bacterial

Mutation

6xHis-TEV-sfGFP-MlaA (227-251; I248N)

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA.3-V5-SPOP (R221C)

Plasmid

#197022

Purpose

Mammalian expression vecor for V5-tagged SPOP

Depositor

Tanja Mittag

Publication

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

R221C

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA.3-V5-SPOP (R354H)

Plasmid

#197023

Purpose

Mammalian expression vecor for V5 tagged SPOP

Depositor

Tanja Mittag

Publication

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

R354H

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RFXANK:JMC029-A02:C213521

Plasmid

#183980

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

RFXANK (RFXANK Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
F0TBY7

Plasmid

#163245

Purpose

Inducible expression of UniProt identifier F0TBY7

Depositor

Martin Engqvist

Publication

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

F0TBY7

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH767

Plasmid

#172172

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WT]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH857

Plasmid

#172175

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Publication

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313_ATP3mut

Plasmid

#120256

Purpose

Expression of ATP3-67 in yeast

Depositor

Markus Ralser

Publication

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Mutation

Isoleucine 304 changed to Asparagine; Alanine 307…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMW-PahZ1 KT-1

Plasmid

#141134

Purpose

Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).

Depositor

Mitch Weiland

Publication

Bolay et al Journal of Chemical Education

Insert

PahZ1

Tags

6x His

Expression

Bacterial

Mutation

Not the full-length gene. PahZ1 fragment starts a…

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Met1 diUb (1-152, R42W)

Plasmid

#110767

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Publication

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Tags

N-His6-3C

Expression

Bacterial

Mutation

R42W

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge