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pCTCON2 Aga2P-HRP

Plasmid

#73152

Purpose

Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

Depositor

Alice Ting

Publication

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Aga2P-HRP

Tags

HA tag and myc tag

Expression

Yeast

Promoter

GAL1

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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FAT FAK biosensor

Plasmid

#78303

Purpose

FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

Depositor

Yingxiao Wang

Publication

Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

Insert

FAT FAK biosensor

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pTKLP-tetA

Plasmid

#71325

Purpose

tetA landing pad template plasmid

Depositor

Thomas Kuhlman

Publication

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBS-Int

Plasmid

#50000

Purpose

Expresses the integrase on a suicide plasmid

Depositor

Brian Robertson, Peter Sander

Publication

Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

Insert

Integrase from pMV361

Expression

Bacterial

Promoter

own

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pKanCMV-mRuby3-18aa-actin

Plasmid

#74255

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to actin

Depositor

Michael Lin

Publication

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

actin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pZ8-T_dCas9

Plasmid

#74062

Purpose

pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

Depositor

Timothy Lu

Publication

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDD315 (mTurquoise2^SEC^2xHA)

Plasmid

#73343

Purpose

mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Publication

Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

Insert

mTurquoise2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

2xHA and C. elegans codon-optimized mTurquoise2

Expression

Worm

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMpGE010

Plasmid

#71536

Purpose

For CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi

Publication

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pET15b His6-FKBPF36V

Plasmid

#73180

Purpose

Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

Depositor

Thomas Wandless

Publication

Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

Insert

FKBP(F36V) (FKBP1A Human)

Tags

His 6x

Expression

Bacterial

Mutation

F36V

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pET28-Dnmt3a3L-sc27

Plasmid

#71827

Purpose

For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

Depositor

Albert Jeltsch

Publication

Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

Insert

Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)

Tags

6xHis tag

Expression

Bacterial

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pFUGW-FerH-ffLuc2-eGFP

Plasmid

#71393

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

Depositor

Glenn Merlino

Publication

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

FerH-ffLuc2-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

FerH

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Publication

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Tags

GFP and Halotag

Expression

Mammalian

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pYLsgRNA-OsU6a

Plasmid

#66194

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6a

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PM-FRB-CFP

Plasmid

#67517

Purpose

FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Tags

CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDestattB (pCM268)

Plasmid

#68313

Purpose

Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

Depositor

Christian Mosimann, Leonard Zon

Publication

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Type

Empty backbone

Use

Zebrafish transgenesis

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMB1610_pRR-Puro

Plasmid

#65853

Purpose

Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Publication

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split puromycin N-acetyltransferase coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Beta-2-adrenergic receptor-mRFP

Plasmid

#67145

Purpose

Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

Depositor

Catherine Berlot

Publication

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mRFP (ADRB2 Discosoma sp., Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-Myc tag

Plasmid

#67617

Purpose

A pCS2 based vector to generate a 3'-Myc tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Publication

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Myc

Expression

Mammalian

Promoter

CMV and SP6

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINB-AMSH*

Plasmid

#66712

Purpose

Expresses activated AMSH in E. coli

Depositor

David Komander

Publication

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

hbp (Stam2 Mouse)
AMSH (STAMBP Human)

Tags

His-3C

Expression

Bacterial

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
pEYFP-Sec31A

Plasmid

#66613

Purpose

YFP fusion of Sec31A

Depositor

David Stephens

Publication

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec31A (SEC31A Human)

Tags

EYFP

Expression

Mammalian

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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